Effect of fumaric acid in combination with or nitrate on in vitro gas production, pH, and redox potential.

Reduction in enteric methane (CH) emissions from cattle can be achieved through use of feed additives, which often results in increased emission of hydrogen (H). The objective of this study was to investigate in vitro effects of a known hydrogen sink, fumaric acid, in combination with either of 2 methane inhibitors, the macroalga or nitrate, on CH and H production, feed degradability, pH, and redox potential. A corn silage (0.5 g; control) was incubated in buffered rumen fluid with the addition of 0.025 g of nitrate (Nit), 0.025 g of dried (Asp), 0.025 g of nitrate + 0.025 g of fumaric acid (Nit+Fum), or 0.025 g of dried + 0.025 g of fumaric acid (Asp+Fum). Accumulated gas production was determined using the Ankom system equipped with airtight gasbags. There were 9 replicates per treatment with 3 replicates per treatment stopped after 24, 36, and 48 h of incubation. The amount of undegraded feed was determined by filtration. Gas composition was determined by gas chromatography. Degradable dry matter, degradable organic matter, pH, redox potential, and gas production data were analyzed using a mixed model. Asp and Asp+Fum reduced CH production by 98% or greater at all incubation times, whereas Nit and Nit+Fum reduced CH production (mL of CH/g of dry matter) by 52% to 63% compared with the control. Hydrogen was only detectable in gas from Asp and Asp+Fum treatments, with no difference in H production between the 2 treatments. The treatments had only minor effects on redox potential in the fermented rumen fluid, and pH was lowest for treatments including In conclusion, both and nitrate reduced CH production. Fumaric acid in combination with did not reduce H production, and treatments including nitrate did not result in any detectable levels of H. Future dose-response in vitro studies will contribute to investigating the potential of fumaric acid as a hydrogen sink during CH mitigation.