鸟类成纤维细胞中 Z 性染色体基因的剂量补偿。

Dosage compensation of Z sex chromosome genes in avian fibroblast cells.

机构信息

International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan.

Regulatory Genomics Research Center, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation.

出版信息

Genome Biol. 2023 Sep 20;24(1):213. doi: 10.1186/s13059-023-03055-z.

DOI:10.1186/s13059-023-03055-z

PMID:37730643

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10510239/

Abstract

In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds.

摘要

在鸟类中，性别是由基因决定的；然而，其分子机制尚不清楚。与哺乳动物的 chrX 不同，禽类的 Z 性染色体（chrZ）缺乏整条染色体失活。为了研究 chrZ 的剂量补偿及其在性别决定中的作用，我们使用一种高度定量的方法，分析了来自七个鸟类物种的雄性和雌性成纤维细胞的转录活性。我们的数据表明，四分之三的 chrZ 基因在鸟类中是严格补偿的，类似于哺乳动物的 chrX。我们还提供了一份完整的未补偿 chrZ 基因列表，并鉴定核糖体蛋白 S6（RPS6）为鸟类中保守的性别二态基因。