Davidson T, Wallace J
Lab Anim. 1986 Oct;20(4):347-50. doi: 10.1258/002367786780808848.
Regional chemotherapy to a tumour is most commonly delivered by intra-arterial infusion. An alternative method of regional drug delivery, isolated perfusion, may be used where anatomical considerations permit. The technique of isolated perfusion in the rabbit hindlimb is described. The use of this model with the implantable rabbit VX2 carcinoma allows estimation of drug uptake by normal tissues, primary tumour and popliteal lymph node metastases. Correlation of such data with blood flow measurements enables targeting of new cytotoxic agents to be evaluated. The effect of perfusate composition on tissue uptake of such an agent, the plant toxin ricin, has been determined.
对肿瘤进行区域化疗最常用的方法是动脉内灌注。在解剖条件允许的情况下，可以采用另一种区域给药方法——隔离灌注。本文描述了兔后肢隔离灌注技术。将这种模型与可植入的兔VX2癌一起使用，可以估计正常组织、原发性肿瘤和腘窝淋巴结转移灶对药物的摄取情况。将这些数据与血流测量结果相关联，能够评估新型细胞毒性药物的靶向性。已经确定了灌注液成分对植物毒素蓖麻毒素这种药物的组织摄取的影响。
