Muckle D S, Dickson J A
Br J Cancer. 1973 Apr;27(4):307-15. doi: 10.1038/bjc.1973.37.
As assessed by decrease in tumour volume and inhibition of tumour cell respiration and glycolysis, hyperthermia (intra-tumour temperature 42°C for one hour) potentiated the destructive effect of radiotherapy (1000 rad) on the allogeneic VX2 carcinoma in the hind limb of rabbits, and chemotherapy (methotrexate) produced a similar potentiation of irradiation. The resulting regression of the primary tumour in each case after dual therapy was comparable to that occurring after 3 applications of local hyperthermia, which has been shown to cure 50% of animals with this carcinoma. Combination therapy did not increase the survival time of the rabbits, however, all of which had lung and lymph node metastases at autopsy. The results focus attention on the relationship between a primary tumour and its metastases. The histological picture and the animal survival data suggest that the mechanism of tumour cell death and resorption of necrotic material following treatment may be important in enabling the host to deal with metastatic cells. After combination therapy, many metabolically and mitotically active cancer cells remained in the tumour mass, and the incomplete destruction of the primary tumour may have left the host with a burden of tumour cells too large to be destroyed by the immune system.
通过肿瘤体积减小以及肿瘤细胞呼吸和糖酵解的抑制来评估，热疗（肿瘤内温度42°C持续1小时）增强了放疗（1000拉德）对兔后肢同种异体VX2癌的破坏作用，化疗（甲氨蝶呤）对放疗也产生了类似的增强作用。在每种情况下，联合治疗后原发肿瘤的消退与3次局部热疗后的消退相当，局部热疗已被证明可治愈50%患有这种癌症的动物。然而，联合治疗并未延长兔子的存活时间，尸检时所有兔子都有肺和淋巴结转移。这些结果将注意力集中在原发肿瘤与其转移灶之间的关系上。组织学图像和动物存活数据表明，治疗后肿瘤细胞死亡和坏死物质吸收的机制可能对宿主应对转移细胞很重要。联合治疗后，肿瘤块中仍有许多代谢和有丝分裂活跃的癌细胞，原发肿瘤的不完全破坏可能使宿主负担的肿瘤细胞数量过多，免疫系统无法将其全部摧毁。
