Durairaj Alwin Beschi, Sivagnanam Ananthi, Monikam Reginald Appavoo, Krishnamoorthy Rajapandiyan, Ahmed Mohammad Z, Alqahtani Ali S, Mydeen Ponnani Kaja
Department of Botany & Research Center, Scott Christian College, Nagercoil - 629003, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli - 627012, Tamil Nadu, India.
Department of Proteomics, Clinbiocare Technology, Tenkasi - 627 814, Tamil Nadu, India.
Iran J Basic Med Sci. 2023;26(10):1202-1211. doi: 10.22038/IJBMS.2023.71125.15450.
The present study evaluated the protein-based analysis to unravel the role and mechanism behind the plant extract treatment in breast cancer cells.
The protein sample was extracted from the cancer cells after treatment with the plant extract and subjected to two-dimensional electrophoresis for protein separation. Further, the proteins that were differentially regulated among the samples which were treated and non-treated were selected and processed further for protein identification using a tandem mass spectrometry approach.
Using these strategies, we identified 16 potential candidates which were showing remarkable changes in treated samples. All the candidates were analyzed further for gene ontology analysis, and it was observed that all proteins were involved in multiple pathways pertaining to the carcinogenesis process. Specifically, apoptotic pathway proteins including BAD, BIK, BID, CASP8, MCL1, BCL2, and BAK1 were highly impacted by treatment with plant extract. All these protein hits were further taken for validation experiments using RT PCR analysis.
Initiation of these apoptotic proteins by plant extract treatment in breast cancer cells shows a positive direction toward nature-based alternative medicine.
本研究评估基于蛋白质的分析方法，以揭示植物提取物治疗乳腺癌细胞背后的作用和机制。
用植物提取物处理癌细胞后提取蛋白质样本，并进行二维电泳以分离蛋白质。此外，选择经处理和未处理样本中差异调节的蛋白质，并用串联质谱法进一步处理以进行蛋白质鉴定。
使用这些策略，我们鉴定出16个潜在候选蛋白，它们在处理后的样本中表现出显著变化。对所有候选蛋白进一步进行基因本体分析，发现所有蛋白质都参与了与致癌过程相关的多个途径。具体而言，包括BAD、BIK、BID、CASP8、MCL1、BCL2和BAK1在内的凋亡途径蛋白受到植物提取物处理的高度影响。所有这些蛋白质靶点都进一步通过RT PCR分析进行验证实验。
植物提取物处理乳腺癌细胞引发这些凋亡蛋白，显示出基于天然的替代医学的积极方向。
