Osei-Mensah Bright, Boakye Yaw Duah, Anyan William Kofi, Agana Theresa Appiah, Aboagye Eugene Agyei, Bentil Ivy, Lomotey Elvis Suatey, Adu Francis, Agyare Christian
Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
J Parasitol Res. 2023 Sep 15;2023:4589424. doi: 10.1155/2023/4589424. eCollection 2023.
Schistosomiasis is a human parasitic disease caused by the species and is recognised in public health as second to malaria in terms of its socioeconomic impact on humans. Four local plants native to many tribes in Ghana and known for their medicinal properties against some diseases were assessed for their cercaricidal activity against cercariae. The plants, namely, stem bark (NLSB), stem bark (SCSB), leaves (MCL), and leaves (OVL), were extracted for their active metabolites using methanol. Preliminary phytochemical screening was carried out on all plant extracts and powdered samples. The crude extracts were tested against cercariae using as the positive control. The percentage of mortalities for each extract was recorded. Gas chromatography/mass spectrometry (GC/MS) analysis was conducted on all the plant extracts. Phytochemical analysis revealed the presence of saponins, glycosides, triterpenoids, sterols, alkaloids, flavonoids, and tannins in almost all the extracts. GC/MS analysis showed the presence of medicinally important active volatile compounds in each extract such as thymol, n-hexadecanoic acid, phytol, and maltol. All four plants showed relatively different levels of activity against cercariae at different times and concentrations. The LC values of the plant extracts were determined at the end of the assay. At 240 min, NLSB, SCSB, MCL, and OVL extracts had LC values of 487.564, 429.898, 197.696, and 0.129 g/mL, respectively. Hence, this study revealed the potency of leaves, leaves, stem bark, and stem bark against . These plants could therefore be exploited as possible candidates for curbing schistosomiasis.
血吸虫病是一种由该物种引起的人类寄生虫病，在公共卫生领域，就其对人类的社会经济影响而言，被认为仅次于疟疾。对加纳许多部落原生的四种当地植物进行了评估，这些植物因对某些疾病具有药用特性而闻名，评估它们对尾蚴的杀尾蚴活性。这些植物分别是[植物名称1]茎皮（NLSB）、[植物名称2]茎皮（SCSB）、[植物名称3]叶（MCL）和[植物名称4]叶（OVL），使用甲醇提取其活性代谢物。对所有植物提取物和粉末样品进行了初步植物化学筛选。以[阳性对照名称]作为阳性对照，对粗提取物进行针对[尾蚴名称]尾蚴的测试。记录每种提取物的死亡率百分比。对所有植物提取物进行了气相色谱/质谱（GC/MS）分析。植物化学分析表明，几乎所有提取物中都含有皂苷、糖苷、三萜类化合物、甾醇、生物碱、黄酮类化合物和单宁。GC/MS分析显示，每种提取物中都存在具有重要药用活性的挥发性化合物，如百里酚、正十六烷酸、叶绿醇和麦芽糖醇。所有四种植物在不同时间和浓度下对[尾蚴名称]尾蚴表现出相对不同程度的活性。在试验结束时测定了植物提取物的半数致死浓度（LC）值。在240分钟时，NLSB、SCSB、MCL和OVL提取物的LC值分别为487.564、429.898、197.696和0.129克/毫升。因此，本研究揭示了[植物名称3]叶、[植物名称4]叶、[植物名称1]茎皮和[植物名称2]茎皮对[尾蚴名称]的效力。因此，这些植物有望被开发为控制血吸虫病的候选药物。
