Van Caenegem Warre, Ceryngier Piotr, Romanowski Jerzy, Pfister Donald H, Haelewaters Danny
Research Group Mycology, Department of Biology, Ghent University, Ghent, Belgium.
Institute of Biological Sciences, Cardinal Stefan Wyszyński University, Warsaw, Poland.
Front Fungal Biol. 2023 Jan 9;3:1040102. doi: 10.3389/ffunb.2022.1040102. eCollection 2022.
Laboulbeniales (Ascomycota, Laboulbeniomycetes) are biotrophic microfungi always attached to the exoskeleton of their arthropod hosts. They do not form hyphae or a mycelium; instead, they undergo determinate growth, developing from a two-celled ascospore to form a multicellular thallus. has been reported on over 30 species of ladybirds (Coleoptera, Coccinellidae); in reality, it represents a complex of species, presumably segregated by host genus association. In this study, we report on thalli on from the Canary Islands and compare them with the described on sp. from Trinidad. We generated the sequences of the internal transcribed spacer (ITS) region, the large subunit (LSU) nuclear ribosomal RNA gene, and the minichromosome maintenance complex component 7 () protein-coding gene. Our phylogenetic reconstruction of based on a concatenated ITS-LSU- dataset revealed sp. ex as a member of the species complex distinct from (s.s.). It also revealed that the sp. ex from Algeria is different from s.s., which is associated with from the USA. This suggests that the species of are not solely segregated by host association, but that there is also a biogeographical component involved. Based on these data, we refrained from referring our material from to . Finally, we discuss the usefulness of as a useful marker for species delimitation in .
虫囊菌目（子囊菌门，虫囊菌纲）是专性活体营养型微真菌，总是附着在其节肢动物宿主的外骨骼上。它们不形成菌丝或菌丝体；相反，它们进行有限生长，从一个双细胞子囊孢子发育形成一个多细胞菌体。已报道在30多种瓢虫（鞘翅目，瓢虫科）上发现该菌；实际上，它代表了一个物种复合体，可能根据宿主属的关联而分离。在本研究中，我们报告了来自加那利群岛的[某种瓢虫]上的菌体，并将它们与特立尼达[某种瓢虫]上描述的[该菌]进行比较。我们生成了内部转录间隔区（ITS）、大亚基（LSU）核糖体RNA基因以及微小染色体维持复合体组分7（MCM7）蛋白编码基因的序列。我们基于串联ITS-LSU-MCM7数据集对[该菌]进行系统发育重建，结果显示来自[某种瓢虫]的[该菌]是与狭义的[该菌]不同的[该菌]物种复合体的成员。它还表明来自阿尔及利亚的来自[某种瓢虫]上的[该菌]与狭义[该菌]不同，狭义[该菌]与来自美国的[某种瓢虫]相关联。这表明[该菌]的物种并非仅根据宿主关联来分离，而是还涉及生物地理成分。基于这些数据，我们避免将来自[某种瓢虫]的材料称为[该菌]。最后，我们讨论了MCM7作为[该菌]物种界定有用标记的实用性。
原文中部分内容缺失具体物种名等关键信息，翻译时用[某种瓢虫]、[该菌]等代替以便完整呈现句子结构。
