Badekova Karakoz, Atazhanova Gayane, Losseva Irina, Medeshova Aigul, Aitkenova Ailazzat, Brazhanova Anar
Institute of Life Science, NC JSC "Karaganda Medical University", Karaganda, Kazakhstan.
J Toxicol. 2023 Sep 14;2023:6691694. doi: 10.1155/2023/6691694. eCollection 2023.
The creation of new herbal medicines for their use in dentistry is relevant. The purpose of this work is to study the acute toxicity of the anticaries dental gel (ACDG3) developed by us based on .
In case of studying the safety of anticaries dental gel "ACDG3" in animals, that with a single dose of up to 2000 mg/kg, the absence of pathological changes in the behavior of animals was noted. Biochemical studies indicate that the studied doses of dental gel did not lead to significant deviations in the blood parameters of mice and deviations fluctuated within the reference values. According to the results of a morphometric study conducted 15 days after the administration of the drug, no deviations were found. The histological evaluation of organs showed little change in the cardiac architecture in animals treated with ACDG3 at doses of 1000 mg/kg and 2000 mg/kg. On the other hand, no significant changes in the cardiac function were observed in all treated mice.
As follows from the results obtained, in case of determining acute toxicity, the studied anticaries gel, ACDG3, showed low toxicity. For mice, LD50 was 2000 mg/kg intragastrically. So, according to the generally accepted classification of the toxicity of substances, ACDG3 can be attributed to the class of low-toxic substances (IV class of toxicity, LD50 > 5000 mg/kg, intragastric administration), that is, to practically nontoxic compounds.
研发用于牙科的新型草药药物具有重要意义。本研究旨在探讨我们研发的基于……的防龋牙科凝胶（ACDG3）的急性毒性。
在研究防龋牙科凝胶“ACDG3”对动物的安全性时，单剂量高达2000mg/kg，未观察到动物行为出现病理变化。生化研究表明，所研究的牙科凝胶剂量未导致小鼠血液参数出现显著偏差，偏差在参考值范围内波动。给药15天后进行的形态计量学研究结果显示，未发现偏差。对器官的组织学评估表明，在1000mg/kg和2000mg/kg剂量下用ACDG3处理的动物心脏结构变化不大。另一方面，在所有接受治疗的小鼠中未观察到心脏功能有显著变化。
根据所得结果，在测定急性毒性时，所研究的防龋凝胶ACDG3显示出低毒性。对于小鼠，经胃内给药的半数致死量（LD50）为2000mg/kg。因此，根据普遍接受的物质毒性分类，ACDG3可归为低毒物质类别（毒性IV类，LD50＞5000mg/kg，经胃内给药），即实际上无毒的化合物。
