Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Department of Pathology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
World J Surg Oncol. 2023 Sep 26;21(1):306. doi: 10.1186/s12957-023-03193-5.
To investigate the relationship between dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) measurements and the potential composition of rectal carcinoma.
Twenty-four patients provided informed consent for this study. DCE-MRI was performed before total mesorectal excision. Quantitative parameters were calculated based on a modified Tofts model. Whole-mount immunohistochemistry and Masson staining sections were generated and digitized at histological resolution. The percentage of tissue components area was measured. Pearson correlation analysis was used to evaluate the correlations between pathological parameters and DCE-MRI parameters.
On the World Health Organization (WHO) grading scale, there were significant differences in extracellular extravascular space (K) (F = 9.890, P = 0.001), mean transit time (MTT) (F = 9.890, P = 0.038), CDX-2 (F = 4.935, P = 0.018), and Ki-67 (F = 4.131, P = 0.031) among G1, G2, and G3. ECV showed significant differences in extramural venous invasion (t = - 2.113, P = 0.046). K was strongly positively correlated with CD34 (r = 0.708, P = 0.000) and moderately positively correlated with vimentin (r = 0.450, P = 0.027). Interstitial volume (Ve) was moderately positively correlated with Masson's (r = 0.548, P = 0.006) and vimentin (r = 0.417, P = 0.043). There was a moderate negative correlation between Ve and CDX-2 (r = - 0.441, P = 0.031). The rate constant from extracellular extravascular space to blood plasma (Kep) showed a strong positive correlation with CD34 expression (r = 0.622, P = 0.001). ECV showed a moderate negative correlation with CDX-2 (r = - 0.472, P = 0.020) and a moderate positive correlation with collagen fibers (r = 0.558, P = 0.005).
The dynamic contrast-enhanced MRI-derived parameters measured in rectal cancer were significantly correlated with the proportion of histological components. This may serve as an optimal imaging biomarker to identify tumor tissue components.
研究动态对比增强（DCE）磁共振成像（MRI）测量值与直肠癌潜在成分之间的关系。
24 名患者同意参与本研究。在全直肠系膜切除术前进行 DCE-MRI 检查。基于改良的 Tofts 模型计算定量参数。生成并以组织学分辨率数字化全组织免疫组化和 Masson 染色切片。测量组织成分面积的百分比。采用 Pearson 相关分析评估病理参数与 DCE-MRI 参数之间的相关性。
在世界卫生组织（WHO）分级中，细胞外细胞外空间（K）（F=9.890，P=0.001）、平均通过时间（MTT）（F=9.890，P=0.038）、CDX-2（F=4.935，P=0.018）和 Ki-67（F=4.131，P=0.031）在 G1、G2 和 G3 之间存在显著差异。ECV 在腔外静脉侵犯（t=-2.113，P=0.046）方面存在显著差异。K 与 CD34 呈强正相关（r=0.708，P=0.000），与波形蛋白呈中度正相关（r=0.450，P=0.027）。间质容积（Ve）与 Masson（r=0.548，P=0.006）和波形蛋白（r=0.417，P=0.043）呈中度正相关。Ve 与 CDX-2 呈中度负相关（r=-0.441，P=0.031）。细胞外细胞外空间至血浆的速率常数（Kep）与 CD34 表达呈强正相关（r=0.622，P=0.001）。ECV 与 CDX-2 呈中度负相关（r=-0.472，P=0.020），与胶原纤维呈中度正相关（r=0.558，P=0.005）。
直肠癌的动态对比增强 MRI 衍生参数与组织成分比例显著相关。这可能是一种最佳的成像生物标志物，可用于识别肿瘤组织成分。
