Gularte Juliana Schons, Sacchetto Lívia, Demoliner Meriane, Girardi Viviane, da Silva Mariana Soares, Filippi Micheli, Pereira Vyctoria Malayhka de Abreu Góes, Hansen Alana Witt, da Silva Luana Letícia, Fleck Juliane Deise, de Almeida Paula Rodrigues, Nogueira Maurício Lacerda, Spilki Fernando Rosado
Universidade Feevale, Laboratório de Microbiologia Molecular, Rodovia ERS-239, n° 2755, Vila Nova, CEP 93525-075, Novo Hamburgo, RS, Brazil.
Faculdade de Medicina de São José do Rio Preto, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Laboratório de Pesquisas em Virologia. Avenida Brigadeiro Faria Lima, 5416, Vila São José, CEP 15090000, São José do Rio Preto, SP, Brazil.
J Clin Virol. 2023 Nov;168:105599. doi: 10.1016/j.jcv.2023.105599. Epub 2023 Sep 21.
Even though Brazil is a country where the dengue virus (DENV) is endemic, until recently, Southern states did not have significant viral circulation, such as Rio Grande do Sul (RS), and some municipalities were even considered dengue-free. During 2022, these places have shown a sharp increase in the incidence of the disease, apparently following a worldwide growth pattern. Therefore, in this study, we monitor and characterize the genetic diversity of DENV circulating in southern Brazil through next-generation sequencing during an outbreak in 2022. We generated 70 DENV-1 genome sequences, all characterized as genotype V, divided into two clade clusters in the L1 lineage. Furthermore, unique mutations have been described in each clade of L1 lineage. Our results are essential in managing outbreaks since these data provide important information during the emergence of DENV circulation in RS. Since the south of Brazil has a lower viral circulation when compared to other Brazilian states, RS still lacks data that can help in understanding the transmission, dissemination, and evolution of the dengue virus. Hence, genomic surveillance efforts are essential to increase the accuracy of preventive actions and to control viral dissemination.
尽管巴西是登革热病毒（DENV）的地方性流行国家，但直到最近，南部各州，如南里奥格兰德州（RS），病毒传播并不显著，一些城市甚至被认为没有登革热。在2022年期间，这些地方的登革热发病率急剧上升，显然遵循全球增长模式。因此，在本研究中，我们通过下一代测序监测并表征了2022年巴西南部登革热病毒爆发期间流行的DENV的遗传多样性。我们生成了70个DENV-1基因组序列，均被鉴定为基因型V，在L1谱系中分为两个进化枝簇。此外，在L1谱系的每个进化枝中都发现了独特的突变。我们的结果对于疫情管理至关重要，因为这些数据在RS地区DENV传播出现时提供了重要信息。由于与巴西其他州相比，巴西南部的病毒传播率较低，RS仍然缺乏有助于了解登革热病毒传播、扩散和进化的数据。因此，基因组监测工作对于提高预防措施的准确性和控制病毒传播至关重要。
