The Ministry of Education Key Laboratory of Clinical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Biosensors (Basel). 2023 Sep 18;13(9):887. doi: 10.3390/bios13090887.
The colonization of some bacteria to their host cell is mediated by selective adhesion between adhesin and glycan. The evaluation of antiadhesive carbohydrates in vitro has great significance in discovering new antibacterial drugs. In this paper, a microfluidic chip integrated with impedimetric neoglycoprotein biosensors was developed to evaluate the antibacterial effect of carbohydrates. Mannosylated bovine serum albumin (Man-BSA) was taken as the neoglycoprotein and immobilized on the microelectrode-modified gold nanoparticles (Au NPs) to form a bionic glycoprotein nanosensing surface (Man-BSA/Au NPs). () was selected as a bacteria model owing to its selective adhesion to the mannose. Electrochemical impedance spectroscopy (EIS) was used to characterize the adhesion capacity of to the Man-BSA/Au NPs and evaluate the antiadhesive efficacy of nine different carbohydrates. It was illustrated that the 4-methoxyphenyl-α-D-pyran mannoside (Phenyl-Man) and mannan peptide (Mannatide) showed excellent antiadhesive efficacy, with IC values of 0.086 mM and 0.094 mM, respectively. The microfluidic device developed in this study can be tested in multiple channels. Compared with traditional methods for evaluating the antibacterial drug in vitro, it has the advantages of being fast, convenient, and cost-effective.
一些细菌对宿主细胞的定殖是通过黏附素和聚糖之间的选择性黏附来介导的。在体外评估抗黏附性碳水化合物对于发现新的抗菌药物具有重要意义。本文开发了一种集成阻抗型糖基化蛋白生物传感器的微流控芯片，用于评估碳水化合物的抗菌效果。甘露糖化牛血清白蛋白（Man-BSA）被用作糖基化蛋白并固定在修饰金纳米颗粒（Au NPs）的微电极上，形成仿生糖蛋白纳米传感表面（Man-BSA/Au NPs）。由于其对甘露糖的选择性黏附，选择作为细菌模型。电化学阻抗谱（EIS）用于表征对 Man-BSA/Au NPs 的黏附能力，并评估九种不同碳水化合物的抗黏附效果。结果表明，4-甲氧基苯基-α-D-吡喃甘露糖苷（Phenyl-Man）和甘露糖肽（Mannatide）表现出优异的抗黏附效果，IC 值分别为 0.086 mM 和 0.094 mM。本研究中开发的微流控装置可以在多个通道中进行测试。与传统的体外评估抗菌药物的方法相比，它具有快速、方便和经济高效的优点。
