Kim Dae-Yun, Hii Jeffrey, Chareonviriyaphap Theeraphap
Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
College of Public Health, Medical & Veterinary Sciences, James Cook University, Brisbane, QLD 4000, Australia.
Insects. 2023 Sep 14;14(9):767. doi: 10.3390/insects14090767.
is a major vector of dengue fever in tropical regions. Spatial repellents (SRs) have shown promise in delaying pesticide resistance. Methods for discriminating concentrations (DCs) are well established using various bioassay tools, while data for high-throughput screening system (HITSS) toxicity bioassay (TOX) are absent. In this study, we compared and optimized lethal (LCs) and sub-lethal concentrations (SLCs) of transfluthrin (TFT) and metofluthrin (MFT) on pyrethroid-susceptible (USDA) and pyrethroid-resistant (Pu-Teuy) (L.) strains, using the HITSS-TOX. Mean mortality (MT) was 100% at LC and DC, compared to LC (45.0 ± 3.7%) and LC (65.8 ± 7.0%) for the USDA strain. However, the resistant strain (Pu-Teuy) showed reduced susceptibility against TFT and a significantly lower MT at LC (12.5 ± 4.4%; t = 5.665, df = 10, < 0.001), LC (9.2 ± 3.5%; t = 4.844, df = 10, = 0.001), LC (55.0 ± 9.9%; t = 4.538, df = 5, = 0.006), and DC (75.0 ± 5.2%; U = 3.0, = 0.007). The DC of TFT (0.15222%) was 4.7-fold higher than for MFT (0.03242%) in USDA strain. The baseline DCs established are useful to better understand susceptibility and the efficacy of various repellents against field populations of .
是热带地区登革热的主要传播媒介。空间驱避剂（SRs）在延缓杀虫剂抗性方面已显示出前景。使用各种生物测定工具，辨别浓度（DCs）的方法已得到充分确立，而高通量筛选系统（HITSS）毒性生物测定（TOX）的数据则不存在。在本研究中，我们使用HITSS-TOX，比较并优化了炔丙菊酯（TFT）和甲氧苄氟菊酯（MFT）对拟除虫菊酯敏感（USDA）和拟除虫菊酯抗性（Pu-Teuy）[（L.）]品系的致死浓度（LCs）和亚致死浓度（SLCs）。在LC和DC时，平均死亡率（MT）为100%，而USDA品系在LC时为（45.0±3.7%），在LC时为（65.8±7.0%）。然而，抗性品系（Pu-Teuy）对TFT的敏感性降低，在LC时MT显著较低（12.5±4.4%；t = 5.665，df = 10，<0.001），在LC时为（9.2±3.5%；t = 4.844，df = 10，= 0.001），在LC时为（55.0±9.9%；t = 4.538，df = 5，= 0.006），在DC时为（75.0±5.2%；U = 3.0，= 0.007）。在USDA品系中，TFT的DC（0.15222%）比MFT（0.03242%）高4.7倍。所确立的基线DCs有助于更好地了解各种驱避剂对野外种群的敏感性和功效。
