Yavasoglu Sare I, Wood Martyn J, Bull James C, Alkış Nergis, Doğan Emrecan, Alkhaibari Abeer M, Butt Tariq M
Department of Biology, Faculty of Science, Aydın Adnan Menderes University, 09010 Aydın, Türkiye.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 73100 Heraklion, Greece.
J Med Entomol. 2025 Jan 13;62(1):39-46. doi: 10.1093/jme/tjae137.
Mosquitoes threaten over half of the world's population through vectored diseases such as malaria, zika, yellow fever, dengue, and chikungunya. Mosquitoes have a highly developed olfactory system attuned to chemotaxis relating to host-seeking, mating, and oviposition behavior. In this study, we aimed to determine the spatial efficacy of 2 plant-based repellent blends (Blend3 and Blend4 that had previously been found to successfully repel Aedes, Anopheles and Culex mosquitoes in wind tunnel assays) in excluding Aedes aegypti from the window entry. A new cage system was developed for parallel "no-choice" and "choice" olfactometric assays. In the no-choice trial, Blends 3 and 4, as well as commercial products (N, N-diethyl-3-methylbenzamide, p-menthane-3,8-diol [PMD], 3-(N-n-butyl-N-acetyl)-amino-propionic acid ethyl ester, and 2-(2-hydroxyethyl)-1-methylpropylstyrene 1-piperidine carboxylate), were adsorbed into filter papers of different sizes and placed in a window created between 2 attached bug dorms. Then, the number of mosquitoes entering the window was counted through a 6-min period. In choice olfactometric assays, Blends 3, 4, and PMD were adsorbed into filter paper and the number of mosquitoes moving away from Blend 3 and PMD were compared. No-choice assays showed that Blend3 (P < 0.001) and Blend4 (P = 0.0012) were more repellent than the best commercial product PMD. Additionally, while Blend 4 was significantly more repellent than Blend 3 (P = 0.012) in the choice assay, overall, these 2 blends show promise as new repellents for the spatial exclusion of Aedes aegypti from window entry alone or as part of a "push-pull'' strategy.
蚊子通过传播疟疾、寨卡病毒、黄热病、登革热和基孔肯雅热等疾病，威胁着世界上超过一半的人口。蚊子拥有高度发达的嗅觉系统，能适应与寻找宿主、交配和产卵行为相关的趋化性。在本研究中，我们旨在确定两种植物基驱蚊混合物（Blend3和Blend4，此前在风洞试验中已发现它们能成功驱避伊蚊、按蚊和库蚊）在阻止埃及伊蚊进入窗户方面的空间效果。我们开发了一种新的笼子系统，用于平行的“无选择”和“有选择”嗅觉测定。在无选择试验中，将Blend3和Blend4以及商业产品（N，N - 二乙基 - 3 - 甲基苯甲酰胺、对薄荷烷 - 3,8 - 二醇[PMD]、3 -（N - 正丁基 - N - 乙酰基） - 氨基丙酸乙酯和2 -（2 - 羟乙基） - 1 - 甲基丙基苯乙烯1 - 哌啶羧酸酯）吸附到不同尺寸的滤纸上，并放置在两个相连的虫舍之间形成的窗户处。然后，在6分钟内统计进入窗户的蚊子数量。在有选择的嗅觉测定中，将Blend3、Blend4和PMD吸附到滤纸上，比较远离Blend3和PMD的蚊子数量。无选择试验表明，Blend3（P < 0.001）和Blend4（P = 0.0012）比最好的商业产品PMD更具驱避性。此外，虽然在有选择试验中Blend4比Blend3的驱避性显著更强（P = 0.012），但总体而言，这两种混合物有望成为新的驱蚊剂，单独用于从窗户入口处空间性地排除埃及伊蚊，或作为“推拉”策略的一部分。
