Donato Tatiani Ayako Goto, Sousa Karolyne Dos Santos Jorge, Kuroda Pedro Akira Bazaglia, Grandini Carlos Roberto
Laboratório de Anelasticidade e Biomateriais, UNESP-Universidade Estadual Paulista, Bauru 17033-360, SP, Brazil.
Institute of Biomaterials, Tribocorrosion and Nanomedicine-IBTN, Bauru 17033-360, SP, Brazil.
J Funct Biomater. 2023 Sep 1;14(9):452. doi: 10.3390/jfb14090452.
This study aimed to produce Ti-15Nb alloy with a low elastic modulus, verify its biocompatibility, and determine whether the alloy indirectly influences cellular viability and morphology, as well as the development of the osteogenic phenotype in cells cultured for 2, 3, and 7 days derived from rat calvarias. Two heat treatments were performed to modify the mechanical properties of the alloy where the Ti-15Nb alloy was heated to 1000 °C followed by slow (-5 °C/min) (SC) and rapid cooling (RC). The results of structural and microstructural characterization (XRD and optical images) showed that the Ti-15Nb alloy was of the α + β type, with slow cooling promoting the formation of the α phase and rapid cooling the formation of the β phase, altering the values for the hardness and elastic modulus. Generally, a more significant amount of the α phase in the Ti-15Nb alloy increased the elastic modulus value but decreased the microhardness value. After the RC treatment, the results demonstrated that the Ti-15Nb alloy did not present cytotoxic effects on the osteogenic cells. In addition, we did not find variations in the cell quantity in the microscopy results that could suggest cell adhesion or proliferation modification.
本研究旨在制备具有低弹性模量的Ti-15Nb合金，验证其生物相容性，并确定该合金是否间接影响细胞活力和形态，以及培养2天、3天和7天的源自大鼠颅骨的细胞中成骨表型的发育。进行了两种热处理以改变合金的力学性能，其中将Ti-15Nb合金加热至1000°C，然后分别进行慢速（-5°C/分钟）冷却（SC）和快速冷却（RC）。结构和微观结构表征（XRD和光学图像）结果表明，Ti-15Nb合金为α + β型，慢速冷却促进α相形成，快速冷却促进β相形成，从而改变了硬度和弹性模量值。一般来说，Ti-15Nb合金中α相含量越高，弹性模量值增加，但显微硬度值降低。RC处理后，结果表明Ti-15Nb合金对成骨细胞没有细胞毒性作用。此外，我们在显微镜检查结果中未发现细胞数量的变化，这表明细胞粘附或增殖没有改变。
