Maehara Shoji, Nakajima Shogo, Watashi Koichi, Agusta Andria, Kikuchi Misato, Hata Toshiyuki, Takayama Kento
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Sanzo,1 Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan.
Department of Virology II, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
J Fungi (Basel). 2023 Sep 5;9(9):905. doi: 10.3390/jof9090905.
Coronavirus disease 2019 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therapeutic agents for the disease are being developed. Endophytes are diverse and produce various secondary metabolites and bioactive substances. We isolated 13 endophytes from the leaves and stems of . Antiviral testing using the culture extracts of these endophytic fungi revealed that five isolates effectively inhibited the replication of SARS-CoV-2. These extracts were used to study the inhibitory effect of SARS-CoV-2 on 3C-like protease, and two isolates proved useful. Both isolates were from the genus ; therefore, the percentage of endophytic fungi in the plant tissue was observed to be an important factor in plant site selection. Thus, we conducted a macroanalysis using next-generation sequencing to analyze the percentage of endophytes in the stems (whole, skin, and inner), leaves, roots, and cultivating soil, as well as to determine the location of each genus. To the best of our knowledge, this study is the first to report that spp. are abundant in stems and that stem-based methods are the most efficient for isolating endophytes targeting spp.
2019冠状病毒病由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起。针对该疾病的治疗药物正在研发中。内生菌种类多样，可产生各种次生代谢产物和生物活性物质。我们从[植物名称]的叶和茎中分离出13株内生菌。使用这些内生真菌的培养提取物进行抗病毒测试发现，5株分离物能有效抑制SARS-CoV-2的复制。这些提取物被用于研究其对SARS-CoV-2 3C样蛋白酶的抑制作用，有2株分离物被证明有效。这两株分离物均来自[属名]属；因此，观察到植物组织中该属内生真菌的比例是植物部位选择的一个重要因素。因此，我们使用二代测序进行了宏分析，以分析茎（整体、外皮和内部）、叶、根和栽培土壤中内生菌的比例，以及确定每个属的位置。据我们所知，本研究首次报道[属名]属在茎中含量丰富，且基于茎的方法是分离靶向[属名]属内生菌最有效的方法。
