Sutera Anna Maria, Arfuso Francesca, Tardiolo Giuseppe, Riggio Valentina, Fazio Francesco, Aiese Cigliano Riccardo, Paytuví Andreu, Piccione Giuseppe, Zumbo Alessandro
Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, Via Palatucci snc, 98168 Messina, Italy.
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Edinburgh EH25 9RG, UK.
Animals (Basel). 2023 May 25;13(11):1750. doi: 10.3390/ani13111750.
This study assessed the potential effect of a co-feed liquid whey-integrated diet on the fecal microbiota of 14 crossbred pigs. The experimental design was as follows: seven pigs were in the control group, fed with a control feed, and seven were in the experimental group, fed with the same control feed supplemented daily with liquid whey. The collection of fecal samples was conducted on each animal before the dietary treatment (T0) and one (T1), and two (T2) months after the beginning of the co-feed integration. In addition, blood samples were collected from each pig at the same time points in order to evaluate the physiological parameters. Taxonomic analysis showed a bacterial community dominated by , , , and phyla that populated the crossbred pig feces. The diversity metrics suggested that the co-feed supplementation affected some alpha diversity indexes of the fecal microbiota. In addition, the differential abundance analysis at the genus level revealed significant differences for various genera, suggesting that the liquid whey supplementation potentially influenced a part of the bacterial community over time. Spearman's correlations revealed that the differential abundant genera identified are positively or negatively correlated with the physiological parameters.
本研究评估了共饲液体乳清综合日粮对14头杂交猪粪便微生物群的潜在影响。实验设计如下：7头猪为对照组，饲喂对照饲料；7头为实验组，饲喂相同的对照饲料，但每天补充液体乳清。在日粮处理前（T0）以及共饲开始后的1个月（T1）和2个月（T2），对每头动物采集粪便样本。此外，在相同时间点从每头猪采集血液样本，以评估生理参数。分类学分析显示，杂交猪粪便中的细菌群落以 、 、 和 门为主。多样性指标表明，共饲补充剂影响了粪便微生物群的一些α多样性指数。此外，属水平的差异丰度分析显示，不同属存在显著差异，表明随着时间的推移，液体乳清补充剂可能影响了一部分细菌群落。斯皮尔曼相关性分析表明，所鉴定的差异丰富属与生理参数呈正相关或负相关。
