Lukova Paolina, Apostolova Elisaveta, Baldzhieva Alexandra, Murdjeva Marianna, Kokova Vesela
Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Plovdiv, 4002 Plovdiv, Bulgaria.
Department of Pharmacology, Toxicology, and Pharmacotherapy, Faculty of Pharmacy, Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria.
Biomedicines. 2023 Sep 11;11(9):2511. doi: 10.3390/biomedicines11092511.
Fucoidans are sulfated polysaccharides detected mainly in the cell walls of brown seaweeds. Here, we examined the effects of single doses of fucoidan derived from (formerly ) on carrageenan-induced paw inflammation in rats. The serum levels of TNF-α, IL-1β, IL-6, and IL-10 of rats with LPS-induced systemic inflammation after 14 days of treatment were also evaluated. Subchronic treatment with fucoidan from attenuated the inflammation during the late phase of the degraded carrageenan-induced paw edema (3rd to 5th hour after carrageenan injection) with peak activity at the 3rd hour after the application. Both doses of fucoidan from (25 and 50 mg/kg bw) significantly decreased the levels of all tested pro-inflammatory cytokines (IL-1β, TNF-α, and IL-6) in the serum of rats with a model of system inflammation but had no effect on the anti-inflammatory cytokine IL-10. The results showed that the repeated application of fucoidan has a more prominent effect on the levels of some pro-inflammatory cytokines in serum in comparison to a single dose of the sulfated polysaccharide. This reveals the potential of fucoidan as an anti-inflammatory agent. Furthermore, fucoidan exhibited antioxidant capacity, determined by 2,2-diphenyl-1-picryl-hydrazyl radical scavenging and ferric reducing antioxidant power assays as follows: IC = 412 µg/mL and 118.72 μM Trolox equivalent/g, respectively.
岩藻依聚糖是主要在褐藻细胞壁中检测到的硫酸化多糖。在此，我们研究了来自（原）的单剂量岩藻依聚糖对大鼠角叉菜胶诱导的爪部炎症的影响。还评估了治疗14天后脂多糖诱导的全身炎症大鼠的血清中TNF-α、IL-1β、IL-6和IL-10的水平。来自的岩藻依聚糖的亚慢性治疗减轻了降解的角叉菜胶诱导的爪部水肿后期（角叉菜胶注射后第3至5小时）的炎症，在应用后第3小时活性达到峰值。来自的两种剂量的岩藻依聚糖（25和50mg/kg体重）均显著降低了系统炎症模型大鼠血清中所有测试的促炎细胞因子（IL-1β、TNF-α和IL-6）的水平，但对抗炎细胞因子IL-10没有影响。结果表明，与单剂量的硫酸化多糖相比，重复应用岩藻依聚糖对血清中某些促炎细胞因子的水平有更显著的影响。这揭示了岩藻依聚糖作为抗炎剂的潜力。此外，岩藻依聚糖表现出抗氧化能力，通过2,2-二苯基-1-苦基肼自由基清除和铁还原抗氧化能力测定如下：IC50分别为412μg/mL和118.72μM Trolox当量/g。
