从中提取的硫酸多糖通过阻断 TLR/NF-Κb 信号转导对巨噬细胞的抗炎作用。

Anti-Inflammatory Effects of Sulfated Polysaccharide from in Macrophages via Blocking TLR/NF-Κb Signal Transduction.

机构信息

Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea.

Department of Chemistry, University of Colombo, Colombo 3, Sri Lanka.

出版信息

Mar Drugs. 2020 Nov 28;18(12):601. doi: 10.3390/md18120601.

This study involves enzymatic extraction of fucoidan from and further purification via ion-exchange chromatography. The chemical and molecular characteristics of isolated fucoidan is evaluated concerning its anti-inflammatory potential in RAW 264.7 macrophages under LPS induced conditions. Structural properties of fucoidan were assessed via FTIR and NMR spectroscopy. NO production stimulated by LPS was significantly declined by fucoidan. This was witnessed to be achieved via fucoidan acting on mediators such as iNOS and COX-2 including pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), with dose dependent down-regulation. Further, the effect is exhibited by the suppression of TLR mediated MyD88, IKK complex, ultimately hindering NF-κB and MAPK activation, proposing its therapeutic applications in inflammation related disorders. The research findings provide an insight in relation to the sustainable utilization of fucoidan from marine brown algae as a potent anti-inflammatory agent in the nutritional, pharmaceutical, and cosmeceutical sectors.

本研究涉及从褐藻中通过酶提取法提取褐藻糖胶，并通过离子交换色谱进一步纯化。根据褐藻糖胶在 LPS 诱导 RAW 264.7 巨噬细胞中的抗炎潜力，评估了分离得到的褐藻糖胶的化学和分子特征。通过傅里叶变换红外光谱（FTIR）和核磁共振（NMR）光谱评估褐藻糖胶的结构特性。褐藻糖胶显著降低了 LPS 刺激的 NO 产生。这被证明是通过褐藻糖胶作用于 iNOS 和 COX-2 等介质以及促炎细胞因子（TNF-α、IL-6 和 IL-1β）来实现的，具有剂量依赖性的下调作用。此外，这种作用还表现为抑制 TLR 介导的 MyD88、IKK 复合物，最终抑制 NF-κB 和 MAPK 的激活，为其在炎症相关疾病中的治疗应用提供了依据。研究结果为海洋褐藻中褐藻糖胶的可持续利用提供了新的思路，为营养、制药和化妆品行业提供了一种有效的抗炎剂。