脱脂核桃壳提取物的体外和体内抗氧化活性及提取条件优化

The Antioxidant Activities In Vitro and In Vivo and Extraction Conditions Optimization of Defatted Walnut Kernel Extract.

作者信息

Zhou Xiaomei, Gong Xiaojian, Li Xu, An Ning, He Jiefang, Zhou Xin, Zhao Chao

机构信息

Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China.

Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang 550001, China.

出版信息

Foods. 2023 Sep 14;12(18):3417. doi: 10.3390/foods12183417.

DOI:10.3390/foods12183417

PMID:37761127

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10528741/

Abstract

The objective of this study was to determine the antioxidant activities of defatted walnut kernel extract (DWE) and whole walnut kernel extract (WE) in vitro and in vivo. Three spectrophotometric methods, DPPH, ABTS, and FRAP, were used in in vitro experiments, and mice were used in in vivo experiments. In addition, response surface methodology (RSM) was used to optimize reflux-assisted ethanol extraction of DWE for maximum antioxidant activity and total phenolic content. The results of in vitro experiments showed that both extracts showed antioxidant activity; however, the antioxidant activity of DWE was higher than that of WE. Both extracts improved the mice's oxidative damage status in in vivo studies. An ethanol concentration of 58%, an extraction temperature of 48 °C, and an extraction time of 77 min were the ideal parameters for reflux-assisted ethanol extraction of DWE. The results may provide useful information for further applications of defatted walnut kernels and the development of functional foods.

摘要

本研究的目的是测定脱脂核桃仁提取物（DWE）和全核桃仁提取物（WE）在体外和体内的抗氧化活性。体外实验采用了三种分光光度法，即DPPH法、ABTS法和FRAP法，体内实验则使用了小鼠。此外，采用响应面法（RSM）优化DWE的回流辅助乙醇提取工艺，以获得最大抗氧化活性和总酚含量。体外实验结果表明，两种提取物均具有抗氧化活性；然而，DWE的抗氧化活性高于WE。在体内研究中，两种提取物均改善了小鼠的氧化损伤状态。乙醇浓度58%、提取温度48℃和提取时间77分钟是DWE回流辅助乙醇提取的理想参数。这些结果可能为脱脂核桃仁的进一步应用和功能性食品的开发提供有用信息。