Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Department of Animal Science, North Carlonina State University, Raleigh, NC 27695, USA.
Genes (Basel). 2023 Sep 12;14(9):1789. doi: 10.3390/genes14091789.
This study aims to collect RNA-Seq data from samples representing dry and lactating mammary tissue, identify lncRNA transcripts, and analyze findings for their features and functional annotation. This allows for connections to be drawn between lncRNA and the lactation process. RNA-Seq data from 103 samples of mammary tissue were gathered from publicly available databases (60 dry, 43 lactating). The samples were filtered to reveal 214 dry mammary lncRNA transcripts and 517 lactating mammary lncRNA transcripts. The lncRNAs met common lncRNA characteristics such as shorter length, fewer exons, lower expression levels, and less sequence conservation when compared to the genome. Interestingly, several lncRNAs showed sequence similarity to genes associated with strong hair keratin intermediate filaments. Human breast cancer research has associated strong hair keratin filaments with mammary tissue cellular resilience. The lncRNAs were also associated with several genes/proteins that linked to pregnancy using expression correlation and gene ontology. Such findings indicate that there are crucial relationships between the lncRNAs found in mammary tissue and the development of the tissue, to meet both the animal's needs and our own production needs; these relationships should be further investigated to ensure that we continue to breed the most resilient, efficient dairy cattle.
本研究旨在从代表干燥和泌乳乳腺组织的样本中收集 RNA-Seq 数据，鉴定 lncRNA 转录本，并对其特征和功能注释进行分析，以确定 lncRNA 与泌乳过程之间的联系。从公共数据库中收集了 103 个乳腺组织样本的 RNA-Seq 数据（60 个干燥，43 个泌乳）。对样本进行过滤，以揭示 214 个干燥乳腺 lncRNA 转录本和 517 个泌乳乳腺 lncRNA 转录本。与基因组相比，lncRNAs 具有较短的长度、较少的外显子、较低的表达水平和较少的序列保守性等常见 lncRNA 特征。有趣的是，一些 lncRNA 与与强毛发角蛋白中间丝相关的基因具有序列相似性。人类乳腺癌研究表明，强毛发角蛋白丝与乳腺组织细胞的韧性有关。通过表达相关性和基因本体分析，这些 lncRNA 还与与妊娠相关的几个基因/蛋白质相关。这些发现表明，乳腺组织中发现的 lncRNA 与组织的发育之间存在着重要的关系，以满足动物和我们自身的生产需求；这些关系应该进一步研究，以确保我们继续培育最有弹性、最高效的奶牛。
