Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.
Center for Physical Sciences and Technology, Saulėtekio av. 3, LT-10257 Vilnius, Lithuania.
Viruses. 2020 Apr 23;12(4):479. doi: 10.3390/v12040479.
A novel cold-adapted siphovirus, vB_PagS_AAS21 (AAS21), was isolated in Lithuania using as the host for phage propagation. AAS21 has an isometric head (85 nm in diameter) and a non-contractile flexible tail (174 × 10 nm). With a genome size of 116,649 bp, bacteriophage AAS21 is the largest -infecting siphovirus sequenced to date. The genome of AAS21 has a G+C content of 39.0% and contains 213 putative protein-encoding genes and 29 genes for tRNAs. A comparative sequence analysis revealed that 89 AAS21 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 63 AAS21 ORFs were functionally annotated, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. Proteomic analysis led to the experimental identification of 19 virion proteins, including 11 that were predicted by bioinformatics approaches. Based on comparative phylogenetic analysis, AAS21 cannot be assigned to any genus currently recognized by ICTV and may represents a new branch of viruses within the family .
一种新型的冷适应噬菌病毒 vB_PagS_AAS21（AAS21）在立陶宛被分离出来，使用 作为噬菌体繁殖的宿主。AAS21 具有等轴的头部（直径约 85nm）和非收缩性的柔性尾部（~174×10nm）。噬菌体 AAS21 的基因组大小为 116649bp，是迄今为止测序的最大的感染性丝状噬菌体。AAS21 的基因组 G+C 含量为 39.0%，包含 213 个推定的蛋白编码基因和 29 个 tRNA 基因。比较序列分析表明，89 个 AAS21 开放阅读框（ORFs）编码独特的蛋白，与数据库条目没有可靠的同一性。总共对 63 个 AAS21 ORFs 进行了功能注释，包括编码负责病毒形态发生、噬菌体-宿主相互作用和 DNA 代谢的蛋白的 ORFs。蛋白质组学分析导致实验鉴定了 19 个病毒粒子蛋白，其中 11 个是通过生物信息学方法预测的。基于比较系统发育分析，AAS21 不能被分配到 ICTV 目前认可的任何属，可能代表了家族 中的病毒的一个新分支。
