Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Centre, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.
Center for Physical Sciences and Technology, Saulėtekio av. 3, LT-10257 Vilnius, Lithuania.
Viruses. 2018 Oct 25;10(11):583. doi: 10.3390/v10110583.
A novel lowtemperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G⁻C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA. A comparative sequence analysis revealed that 46 out of 99 Vid5 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 33 Vid5 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phagehost interactions, and DNA metabolism. In addition, a cluster of genes possibly involved in the biosynthesis of 7-deazaguanine derivatives was identified. Notably, one of these genes encodes a putative preQ₀/preQ₁ transporter, which has never been detected in bacteriophages to date. A proteomic analysis led to the experimental identification of 11 virion proteins, including nine that were predicted by bioinformatics approaches. Based on the phylogenetic analysis, Vid5 cannot be assigned to any genus currently recognized by ICTV, and may represent a new one within the family of .
一种新型低温长尾噬菌体，vB_PagS_Vid5（Vid5），在立陶宛使用分离株进行噬菌体繁殖时被分离出来。Vid5 的基因组长 61437bp，G-C 含量为 48.8%，包含 99 个可能的蛋白质编码基因和一个 tRNA 基因。比较序列分析显示，99 个 Vid5 开放阅读框（ORF）中有 46 个编码独特的蛋白质，与数据库条目没有可靠的同一性。总共，33 个 Vid5 ORF 被赋予了推测的功能注释，包括编码负责病毒形态发生、噬菌体-宿主相互作用和 DNA 代谢的蛋白质的那些。此外，还鉴定了可能参与 7-脱氮鸟嘌呤衍生物生物合成的基因簇。值得注意的是，其中一个基因编码一个假定的前 Q₀/前 Q₁ 转运蛋白，迄今为止在噬菌体中从未检测到过。蛋白质组学分析导致实验鉴定了 11 种病毒蛋白，其中 9 种是通过生物信息学方法预测的。根据系统发育分析，Vid5 不能归入 ICTV 目前认可的任何属，可能代表长尾噬菌体科中的一个新属。
