Žukauskienė Emilija, Šimoliūnienė Monika, Truncaitė Lidija, Skapas Martynas, Kaupinis Algirdas, Valius Mindaugas, Meškys Rolandas, Šimoliūnas Eugenijus
Life Sciences Centre, Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.
Center for Physical Sciences and Technology, Saulėtekio av. 3, LT-10257 Vilnius, Lithuania.
Microorganisms. 2021 Mar 23;9(3):668. doi: 10.3390/microorganisms9030668.
A cold-adapted siphovirus, vB_PagS_AAS23 (AAS23) was isolated in Lithuania using the strain AUR for the phage propagation. The double-stranded DNA genome of AAS23 (51,170 bp) contains 92 probable protein encoding genes, and no genes for tRNA. A comparative sequence analysis revealed that 25 of all AAS23 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. Based on the phylogenetic analysis, AAS23 has no close relationship to other viruses publicly available to date and represents a single species of the genus within the family . The phage is able to form plaques in bacterial lawns even at 4 °C and demonstrates a depolymerase activity. Thus, the data presented in this study not only provides the information on -infecting bacteriophages, but also offers novel insights into the diversity of cold-adapted viruses and their potential to be used as biocontrol agents.
一种冷适应的肌尾噬菌体vB_PagS_AAS23（AAS23）在立陶宛使用AUR菌株进行噬菌体增殖时被分离出来。AAS23的双链DNA基因组（51,170 bp）包含92个可能的蛋白质编码基因，且没有tRNA基因。比较序列分析表明，AAS23的所有开放阅读框（ORF）中有25个编码的独特蛋白质与数据库条目没有可靠的同源性。基于系统发育分析，AAS23与目前公开的其他病毒没有密切关系，代表了该科内一个属的单一物种。该噬菌体即使在4°C时也能在细菌菌苔上形成噬菌斑，并表现出解聚酶活性。因此，本研究提供的数据不仅提供了有关感染噬菌体的信息，还为冷适应病毒的多样性及其作为生物防治剂的潜力提供了新的见解。
