Scientific Research Division, 4Life Research, Sandy, UT 84070, USA.
Life Length SL, 28010 Madrid, Spain.
Int J Mol Sci. 2023 Sep 19;24(18):14273. doi: 10.3390/ijms241814273.
Telomerase activity coincides with lengthening of the ends of chromosomes known as telomeres. Telomere length is used as a marker for cellular aging. Telomeres shorten over time as cells divide, and certain bioactive compounds such as gold nanoparticles (AuNPs) may slow the shortening of telomeres by increasing telomerase activity. The objective of the present study is to assess the effect of AuNPs on telomerase activity and telomere length in human fibroblasts. Telomerase activity was measured using enzyme-linked immunosorbent assay (ELISA) in primary human lung fibroblasts (IMR90) and using quantitative PCR-based telomeric repeat amplification protocol (Q-TRAP) in primary human dermal fibroblasts, neonatal (HDFn). Telomere length was determined by Telomere Analysis Technology (TAT)assay in HDFn. In IMR90, all AuNP treatments showed significant increases in telomerase activity when compared to earlier passages. HDFn treated with AuNPs at 0 ppm, 0.05 ppm, 0.5 ppm, or 5 ppm did not show significant differences in telomerase activity compared to the control group. Significant differences in telomere length in HDFn were observed at 2 weeks of 0.05 and 0.5 ppm AuNPs under oxidative culture conditions as compared to the control group. The study showed preliminary evidence that AuNPs may increase telomerase activity and decelerate the shortening of telomeres in human fibroblasts, suggesting its potential anti-aging effects, which warrants further investigation.
端粒酶活性与染色体末端端粒的延长相一致。端粒长度被用作细胞衰老的标志物。随着细胞分裂，端粒会随着时间的推移而缩短，而某些生物活性化合物，如金纳米粒子（AuNPs），可能通过增加端粒酶活性来减缓端粒缩短的速度。本研究的目的是评估 AuNPs 对人成纤维细胞中端粒酶活性和端粒长度的影响。使用酶联免疫吸附测定（ELISA）测量原代人肺成纤维细胞（IMR90）中的端粒酶活性，使用基于定量 PCR 的端粒重复扩增协议（Q-TRAP）测量原代人皮肤成纤维细胞、新生儿（HDFn）中的端粒酶活性。通过端粒分析技术（TAT）测定 HDFn 中的端粒长度。在 IMR90 中，与早期传代相比，所有 AuNP 处理均显示端粒酶活性显著增加。与对照组相比，在 0 ppm、0.05 ppm、0.5 ppm 或 5 ppm AuNPs 处理的 HDFn 中端粒酶活性没有显著差异。在氧化培养条件下，与对照组相比，在 0.05 和 0.5 ppm AuNPs 作用 2 周时，HDFn 中端粒长度出现显著差异。该研究初步表明，AuNPs 可能增加人成纤维细胞中端粒酶活性并减缓端粒缩短，表明其具有潜在的抗衰老作用，值得进一步研究。
