Yan Zhuohong, Wang Jinghui, Pang Yu, Wang Xiaojue, Yi Ling, Wei Panjian, Ruan Hongyun, Gu Meng, Zhang Hongtao, Yang Xinting
Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Department of Medical Oncology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Microorganisms. 2023 Sep 8;11(9):2259. doi: 10.3390/microorganisms11092259.
Tuberculous pleurisy (TP) is one of the most common forms of extrapulmonary tuberculosis, but its diagnosis is challenging. Lipoarabinomannan (LAM) antigen is a biomarker for (Mtb) infection. LAM detection has potential as an auxiliary diagnostic method for TP. We have successfully generated five rabbit anti-LAM monoclonal antibodies (BJRbL01, BJRbL03, BJRbL20, BJRbL52, and BJRbL76). Here, anti-LAM antibodies were tested to detect LAM in the pleural fluid and plasma of patients with TP by sandwich enzyme-linked immunosorbent assays (ELISAs). The results revealed that all of the anti-LAM antibodies were successfully used as capture and detection antibodies in sandwich ELISAs. The BJRbL01/BJRbL01-Bio pair showed better performance than the other antibody pairs for detecting mycobacterial clinical isolates and had a limit of detection of 62.5 pg/mL for purified LAM. LAM levels were significantly higher in the pleural fluid and plasma of patients with TP than in those of patients with malignant pleural effusion or the plasma of non-TB, and LAM levels in the pleural fluid and plasma were positively correlated. Moreover, LAM levels in the pleural fluid sample were significantly higher in confirmed TP patients than in clinically diagnosed TP patients. Our studies provide novel LAM detection choices in the pleural fluid and plasma of TP patients and indicate that LAM detection assay has an auxiliary diagnostic value for TP, which may help to improve the diagnosis of TP.
结核性胸膜炎（TP）是肺外结核最常见的形式之一，但其诊断具有挑战性。脂阿拉伯甘露聚糖（LAM）抗原是结核分枝杆菌（Mtb）感染的生物标志物。LAM检测作为TP的辅助诊断方法具有潜力。我们已成功制备了五种兔抗LAM单克隆抗体（BJRbL01、BJRbL03、BJRbL20、BJRbL52和BJRbL76）。在此，通过夹心酶联免疫吸附测定（ELISA）检测抗LAM抗体，以检测TP患者胸腔积液和血浆中的LAM。结果显示，所有抗LAM抗体均成功用作夹心ELISA中的捕获抗体和检测抗体。BJRbL01/BJRbL01-Bio组合在检测分枝杆菌临床分离株方面表现优于其他抗体组合，对纯化LAM的检测限为62.5 pg/mL。TP患者胸腔积液和血浆中的LAM水平显著高于恶性胸腔积液患者或非结核患者的血浆，且胸腔积液和血浆中的LAM水平呈正相关。此外，确诊的TP患者胸腔积液样本中的LAM水平显著高于临床诊断的TP患者。我们的研究为TP患者的胸腔积液和血浆提供了新的LAM检测选择，并表明LAM检测对TP具有辅助诊断价值，这可能有助于改善TP的诊断。
