Ershov Alexey P, Babich Tamara L, Grouzdev Denis S, Sokolova Diyana S, Semenova Ekaterina M, Avtukh Alexander N, Poltaraus Andrey B, Ianutsevich Elena A, Nazina Tamara N
Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
SciBear OU, 10115 Tallinn, Estonia.
Microorganisms. 2023 Sep 14;11(9):2314. doi: 10.3390/microorganisms11092314.
The current work deals with genomic analysis, possible ecological functions, and biotechnological potential of two bacterial strains, HO-A22 and SHC 2-14, isolated from unique subsurface environments, the Cheremukhovskoe oil field (Tatarstan, Russia) and nitrate- and radionuclide-contaminated groundwater (Tomsk region, Russia), respectively. New isolates were characterized using polyphasic taxonomy approaches and genomic analysis. The genomes of the strains HO-A22 and SHC 2-14 contain the genes involved in nitrate reduction, hydrocarbon degradation, extracellular polysaccharide synthesis, and heavy metal detoxification, confirming the potential for their application in various environmental biotechnologies. Genomic data were confirmed by cultivation studies. Both strains were found to be neutrophilic, chemoorganotrophic, facultatively anaerobic bacteria, growing at 15-33 °C and 0-1.6% NaCl (/). The 16S rRNA gene sequences of the strains were similar to those of the type strains of the genus (99.0-100.0%). Nevertheless, genomic characteristics of strain HO-A22 were below the thresholds for species delineation: the calculated average nucleotide identity (ANI) values were 83.7-92.4% (<95%), and digital DNA-DNA hybridization (dDDH) values were within the range of 25.4-45.9% (<70%), which supported our conclusion that HO-A22 (=VKM B-3646 = KCTC 92427) represented a novel species of the genus , with the proposed name sp. nov. Strain SHC 2-14 was assigned to the species '', which has not been validly published. This study expanded the knowledge about the phenotypic diversity among members of the genus and its potential for the biotechnologies of oil recovery and radionuclide pollution treatment.
当前的研究工作涉及从独特的地下环境中分离出的两株细菌HO - A22和SHC 2 - 14的基因组分析、可能的生态功能及生物技术潜力，这两株菌分别来自俄罗斯鞑靼斯坦的切列穆霍夫斯科耶油田和俄罗斯托木斯克地区受硝酸盐和放射性核素污染的地下水。使用多相分类方法和基因组分析对新分离菌株进行了表征。HO - A22和SHC 2 - 14菌株的基因组包含参与硝酸盐还原、碳氢化合物降解、细胞外多糖合成和重金属解毒的基因，证实了它们在各种环境生物技术中的应用潜力。通过培养研究证实了基因组数据。发现这两株菌均为嗜中性、化能有机营养型、兼性厌氧细菌，在15 - 33°C和0 - 1.6% NaCl（/）条件下生长。这些菌株的16S rRNA基因序列与该属模式菌株的序列相似（99.0 - 100.0%）。然而，HO - A22菌株的基因组特征低于物种划分的阈值：计算得到的平均核苷酸同一性（ANI）值为83.7 - 92.4%（<95%），数字DNA - DNA杂交（dDDH）值在25.4 - 45.9%范围内（<70%），这支持了我们的结论，即HO - A22（=VKM B - 3646 = KCTC 92427）代表该属的一个新物种，提议命名为sp. nov.。SHC 2 - 14菌株被归为“”物种，该物种尚未有效发表。本研究扩展了关于该属成员间表型多样性及其在石油开采和放射性核素污染处理生物技术方面潜力的认识。
