Ul Islam Basharat, Mir Saba, Dar Mohammad Saleem, Khan Gazala H, Shikari Asif B, Sofi Najeeb Ul Rehman, Mohiddin Fayaz, Ahangar Mohammad Ashraf, Jehangir Intikhab Aalum, Kumar Satish, Singh Gyanendra, Wani Shabir H
Division of Genetics and Plant Breeding, Faculty of Agriculture, Wadura, Sopore, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar 190025, Jammu and Kashmir, India.
Mountain Research Centre for Field Crops, Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar 190025, Jammu and Kashmir, India.
Plants (Basel). 2023 Sep 12;12(18):3239. doi: 10.3390/plants12183239.
Wheat is highly affected by stripe rust disease, particularly under cooler environments, and the losses can reach up to 100 percent depending on the intensity of infection and the susceptibility of the genotype. The most effective method to manage this disease is the use of resistant varieties. In the present study, 192 wheat genotypes were evaluated for stripe rust resistance under field conditions and also in a laboratory using molecular markers. These lines included pre-breeding germplasm developed for rust resistance and some high-yielding commercially grown wheat varieties. Out of 192 genotypes, 53 were found to be resistant, and 29 showed moderate resistance reaction under field conditions, whereas the remaining genotypes were all either moderately susceptible or susceptible. Under controlled conditions, out of 109 genotypes, only 12 were found to be resistant to all the six virulent/pathogenic pathotypes. Additionally, a selection of 97 genotypes were found resistant in field screening and were subjected to molecular validation using the markers linked to major R-genes, ., , , and . Nine genotypes possessed the gene, twelve had the gene, fourteen had the gene and thirty-two had the gene. The resistance genes studied in the current study are effective in conferring resistance against stripe rust disease. The genotypes identified as resistant under both field and controlled conditions can be used as sources in stripe rust resistance breeding programs.
小麦极易受到条锈病的影响，尤其是在较凉爽的环境下，根据感染强度和基因型的易感性，损失可达100%。防治这种病害最有效的方法是使用抗病品种。在本研究中，对192个小麦基因型进行了田间条件下以及实验室中使用分子标记的条锈病抗性评估。这些品系包括为抗锈病培育的预育种种质以及一些高产的商业种植小麦品种。在192个基因型中，有53个被发现具有抗性，29个在田间条件下表现出中度抗性反应，而其余基因型均为中度易感或易感。在可控条件下，在109个基因型中，只有12个被发现对所有六种毒性/致病致病型均具有抗性。此外，在田间筛选中发现97个基因型具有抗性，并使用与主要R基因连锁的标记进行分子验证，即 、 、 、 和 。9个基因型具有 基因，12个具有 基因，14个具有 基因，32个具有 基因。本研究中所研究的抗性基因在赋予条锈病抗性方面是有效的。在田间和可控条件下均被鉴定为抗性的基因型可作为条锈病抗性育种计划的种质来源。
