Department of Plant Pathology, PAU, Ludhiana, India.
Department of Plant Breeding and Genetics, PAU, Ludhiana, India.
Sci Rep. 2023 Jun 2;13(1):9007. doi: 10.1038/s41598-023-36197-y.
Thirteen known Yr gene-associated markers pertaining to genes (Yr5, Yr10, Yr15, Yr24/Yr26) were used to identify the genes in selected wheat germplasm which were found resistant under field conditions at two locations in Punjab, India against stripe rust. In field evaluation, 38 genotypes exhibited highly resistant response, with a final rust severity (FRS) ranging from 0 to TR. Seven genotypes expressed a resistant to moderately resistant response with FRS ranging from 5MR-10S. In race-specific phenotying against most prevalent pathotypes of Puccinia striiformis tritici (46S119,110S119 &238S119) by seedling reaction test (SRT) 14 genotypes (29.2%) were found to be immune (IT = 0), 28 genotypes (58.3%) were resistant (IT = 1), and 3 genotypes (6.3%) were moderately resistant (IT = 2). Yr5 was detected in sixteen lines with the help of two markers Xwmc175 and Xgwm120 linked with Yr5. Yr10 was detected in ten lines with the marker Xpsp3000 and Yr15 was detected in fourteen lines with two linked markers; Xgwm413 and Xgwm273. Likewise, Yr24/26 was detected in 15 lines with two linked markers, namely Xbarc181 and Xbarc187. Based on the race specific phenotyping data and marker data, fourteen lines were found to carry a single gene, 16 showed the presence of two gene combinations, and seven genotypes were found to have a combination of three genes. Frequencies of Yr5, Yr15 and Yr26/Yr24 was high among test wheat germplasm in comparison to Yr10.
利用与基因（Yr5、Yr10、Yr15、Yr24/Yr26）相关的 13 个已知 Yr 基因标记，鉴定了在印度旁遮普邦两个地点的田间条件下对条锈病表现出抗性的选定小麦种质中的基因。在田间评价中，38 个基因型表现出高度抗性反应，最终锈病严重度（FRS）范围为 0 到 TR。7 个基因型表现出抗性到中度抗性反应，FRS 范围为 5MR-10S。在针对小麦条锈菌最普遍的生理小种（46S119、110S119 和 238S119）的种传鉴定中，通过幼苗反应测试（SRT）发现 14 个基因型（29.2%）为免疫（IT=0），28 个基因型（58.3%）为抗性（IT=1），3 个基因型（6.3%）为中度抗性（IT=2）。借助与 Yr5 连锁的两个标记 Xwmc175 和 Xgwm120，在 16 个品系中检测到 Yr5。在 10 个品系中检测到 Yr10，使用标记 Xpsp3000，在 14 个品系中检测到 Yr15，使用两个连锁标记 Xgwm413 和 Xgwm273。同样，在 15 个品系中检测到 Yr24/26，使用两个连锁标记 Xbarc181 和 Xbarc187。基于种传鉴定数据和标记数据，发现 14 个品系携带单个基因，16 个品系显示存在两个基因组合，7 个基因型显示存在三个基因组合。与 Yr10 相比，在测试的小麦种质中，Yr5、Yr15 和 Yr26/Yr24 的频率较高。
