The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UK.
Oxford Vaccine Group, Centre for Clinical Vaccinology and Tropical Medicine (CCVTM), Churchill Hospital Old Road, Headington, Oxford OX3 7LE, UK.
Viruses. 2023 Sep 5;15(9):1876. doi: 10.3390/v15091876.
Nairobi sheep disease (NSD), caused by the viral agent NSD virus (NSDV), is a haemorrhagic fever disease affecting and inducing high mortality in sheep and goat populations. NSDV belongs to the genus of the family from the order . Other viruses circulating in livestock such as Crimean-Congo haemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV) are members of the same genus and are reported to share antigenic features. There are very few available materials to study NSDV infection both in vitro and in vivo. In the present work, we characterised two monoclonal antibodies generated in mice that recognise NSDV specifically but not CCHFV or DUGV, along with a potential use to define virus-infected cells, using flow cytometry. We believe this tool can be useful for research, but also NSDV diagnostics, especially through immunological staining.
内罗毕绵羊病（NSD）由病毒剂 NSD 病毒（NSDV）引起，是一种影响绵羊和山羊种群并导致高死亡率的出血热疾病。NSDV 属于 科 的 属。在牲畜中循环的其他病毒，如克里米亚-刚果出血热病毒（CCHFV）和 Dugbe 病毒（DUGV），也是同一属的成员，并被报道具有共同的抗原特征。目前，用于研究 NSVD 感染的材料非常有限，无论是在体外还是体内。在本工作中，我们鉴定了两种在小鼠中产生的单克隆抗体，它们特异性识别 NSDV，但不识别 CCHFV 或 DUGV，同时还可以使用流式细胞术来定义病毒感染的细胞。我们相信该工具不仅对研究有用，而且对 NSDV 的诊断也有用，特别是通过免疫染色。
