The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom.
Vet Res. 2012 Oct 19;43(1):71. doi: 10.1186/1297-9716-43-71.
Partly due to climate change, and partly due to changes of human habitat occupation, the impact of tick-borne viruses is increasing. Nairobi sheep disease virus (NSDV) and Ganjam virus (GV) are two names for the same virus, which causes disease in sheep and goats and is currently known to be circulating in India and East Africa. The virus is transmitted by ixodid ticks and causes a severe hemorrhagic disease. We have developed a real-time PCR assay for the virus genome and validated it in a pilot study of the pathogenicity induced by two different isolates of NSDV/GV. One isolate was highly adapted to tissue culture, grew in most cell lines tested, and was essentially apathogenic in sheep. The second isolate appeared to be poorly adapted to cell culture and retained pathogenicity in sheep. The real-time PCR assay for virus easily detected 4 copies or less of the viral genome, and allowed a quantitative measure of the virus in whole blood. Measurement of the changes in cytokine mRNAs showed similar changes to those observed in humans infected by the closely related virus Crimean Congo hemorrhagic fever virus.
由于气候变化和人类栖息地占用的变化，蜱传病毒的影响正在增加。内罗毕绵羊病病毒（NSDV）和甘贾姆病毒（GV）是同一种病毒的两个名称，它可引起绵羊和山羊发病，目前已知在印度和东非流行。该病毒通过硬蜱传播，引起严重的出血性疾病。我们已经开发出一种针对该病毒基因组的实时 PCR 检测方法，并在对两种不同 NSVD/GV 分离株引起的致病性的初步研究中对其进行了验证。一种分离株高度适应组织培养，在大多数测试的细胞系中生长，对绵羊基本无致病性。第二种分离株似乎对细胞培养的适应性较差，对绵羊仍具有致病性。用于检测病毒的实时 PCR 检测方法可以轻松检测到少于 4 个拷贝的病毒基因组，并可对全血中的病毒进行定量测量。细胞因子 mRNA 的测量结果显示出与人类感染密切相关的克里米亚-刚果出血热病毒相似的变化。
