Joshi Akshay, Singh Neetu
Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, Delhi 110016, India.
Biomedical Engineering Unit, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, Delhi 110029, India.
ACS Omega. 2023 Sep 13;8(38):34249-34261. doi: 10.1021/acsomega.3c02713. eCollection 2023 Sep 26.
Cells inside the body are embedded into a highly structured microenvironment that consists of cells that lie in direct or close contact with other cell types that regulate the overall tissue function. Therefore, coculture models are versatile tools that can generate tissue engineering constructs with improved mimicking of conditions. While there are many reviews that have focused on pattering a single cell type, very few reviews have been focused on techniques for coculturing multiple cell types on a single substrate with precise control. In this regard, this Review covers various technologies that have been utilized for the development of these patterned coculture models while mentioning the limitations associated with each of them. Further, the application of these models to various tissue engineering applications has been discussed.
体内细胞嵌入高度结构化的微环境中，该微环境由与其他调节整体组织功能的细胞类型直接或紧密接触的细胞组成。因此，共培养模型是多功能工具，可生成更能模拟实际条件的组织工程构建体。虽然有许多综述专注于单一细胞类型的图案化，但很少有综述关注在单一基质上精确控制共培养多种细胞类型的技术。在这方面，本综述涵盖了用于开发这些图案化共培养模型的各种技术，同时提及了每种技术的局限性。此外，还讨论了这些模型在各种组织工程应用中的应用。
