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接受体外受精的多囊卵巢综合征患者新鲜与冷冻胚胎培养基中的线粒体DNA：成功妊娠的一种可能预测标志物

Mitochondrial DNA in Fresh versus Frozen Embryo Culture Media of Polycystic Ovarian Syndrome Patients Undergoing Invitro Fertilization: A Possible Predictive Marker of a Successful Pregnancy.

作者信息

Sayed Ghadir A, Al-Sawaf Hussein A, Al-Sawaf Ahmed H, Saeid Mohamed, Maged Ahmed, Ibrahim Iman Hassan

机构信息

Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt.

Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University, Cairo, Egypt.

出版信息

Pharmgenomics Pers Med. 2021 Jan 11;14:27-38. doi: 10.2147/PGPM.S284064. eCollection 2021.

DOI:10.2147/PGPM.S284064
PMID:33469340
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7810675/
Abstract

PURPOSE

Frozen embryos transfer (ET) may improve the live-birth and reduce rates of ovarian hyperstimulation in polycystic ovary syndrome (PCOS) patients. Morphological criteria are the classical way for embryo selection, yet recently, many biochemical and genetic markers have been developed. This study aimed to compare fresh and frozen ET using the mtDNA/gDNA ratio of embryo secretome and the possibility of using this ratio as a predictive marker of PCOS pregnancy rate.

SUBJECTS AND METHODS

One hundred PCOS patients undergoing IVF were chosen according to Rotterdam criteria and divided into two groups. Group I (50 with fresh ET), group II (50 with frozen ET), and otherwise 33 apparently healthy women as a control group with fresh ET. We then carried out absolute quantification of embryo culture media mtDNA and gDNA by real-time PCR.

RESULTS

mtDNA/gDNA ratio was significantly low in PCOS embryo culture media in comparison with control. Additionally, while the mtDNA/gDNA ratio was significantly high in pregnant PCOS embryo culture media, it was high, though not statistically significant, in the fresh ET than frozen ET group. mtDNA/gDNA ratio sensitivity and specificity in PCOS embryo culture media as a predictive value of pregnancy rate were (86% and 96%, respectively).

CONCLUSION

mtDNA/gDNA ratio measurement in PCOS embryo culture media is a novel marker that can be clinically applied as a predictive value of the quality of the morphologically good embryo.

摘要

目的

冷冻胚胎移植（ET）可能会提高多囊卵巢综合征（PCOS）患者的活产率并降低卵巢过度刺激的发生率。形态学标准是胚胎选择的经典方法，但最近已开发出许多生化和基因标志物。本研究旨在比较使用胚胎分泌组的线粒体DNA/基因组DNA（mtDNA/gDNA）比率进行新鲜胚胎移植和冷冻胚胎移植，并探讨将该比率用作PCOS妊娠率预测标志物的可能性。

受试者与方法

根据鹿特丹标准选择100例接受体外受精（IVF）的PCOS患者，分为两组。第一组（50例进行新鲜胚胎移植），第二组（50例进行冷冻胚胎移植），另外选取33名明显健康的女性作为新鲜胚胎移植的对照组。然后通过实时聚合酶链反应（PCR）对胚胎培养基中的mtDNA和基因组DNA进行绝对定量。

结果

与对照组相比，PCOS胚胎培养基中的mtDNA/gDNA比率显著降低。此外，虽然PCOS妊娠胚胎培养基中的mtDNA/gDNA比率显著升高，但新鲜胚胎移植组的该比率虽高于冷冻胚胎移植组，但差异无统计学意义。PCOS胚胎培养基中mtDNA/gDNA比率作为妊娠率预测值的敏感性和特异性分别为86%和96%。

结论

测量PCOS胚胎培养基中的mtDNA/gDNA比率是一种新型标志物，可在临床上用作形态良好胚胎质量的预测值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/5f19cd066403/PGPM-14-27-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/d88e254db873/PGPM-14-27-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/58e81d234cb4/PGPM-14-27-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/87d4b1c64d47/PGPM-14-27-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/ecd9978050ae/PGPM-14-27-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/59dfd316c00c/PGPM-14-27-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/99fe53bff344/PGPM-14-27-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/5f19cd066403/PGPM-14-27-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/d88e254db873/PGPM-14-27-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/58e81d234cb4/PGPM-14-27-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/87d4b1c64d47/PGPM-14-27-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/ecd9978050ae/PGPM-14-27-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/59dfd316c00c/PGPM-14-27-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/99fe53bff344/PGPM-14-27-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1b/7810675/5f19cd066403/PGPM-14-27-g0007.jpg

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