Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Front Cell Infect Microbiol. 2023 Sep 15;13:1265027. doi: 10.3389/fcimb.2023.1265027. eCollection 2023.
There is an urgent need to develop therapeutic options for biofilm-producing Staphylococcus aureus (S. aureus). Therefore, the renewed interest in essential oils (EOs), especially carvacrol, linalool and eugenol, has attracted the attention of our research group.
Multidrug resistance and multivirulence profiles in addition to biofilm production of S. aureus strains isolated from cows with mastitis were evaluated using both phenotypic and genotypic methods. The antimicrobial and antibiofilm activities of EOs were tested using both in vitro and molecular docking studies. Moreover, the interactions between commonly used antibiotics and the tested EOs were detected using the checkerboard method.
We found that all our isolates (n= 37) were biofilm methicillin resistant S. aureus (MRSA) producers and 40.5% were vancomycin resistant S. aureus (VRSA). Unfortunately, 73 and 43.2% of the recovered MRSA isolates showed multidrug resistant (MDR) and multivirulence patterns, respectively. The antimicrobial activities of the tested EOs matched with the phenotypic evaluation of the antibiofilm activities and molecular docking studies. Linalool showed the highest antimicrobial and antibiofilm activities, followed by carvacrol and eugenol EOs. Fortunately, synergistic interactions between the investigated EOs and methicillin or vancomycin were detected with fractional inhibitory concentration index (FICI) values ≤ 0.5. Moreover, the antimicrobial resistance patterns of 13 isolates changed to sensitive phenotypes after treatment with any of the investigated EOs. Treatment failure of bovine mastitis with resistant S. aureus can be avoided by combining the investigated EOs with available antimicrobial drugs.
We hope that our findings can be translated into a formulation of new pharmaceutical dosage forms against biofilm-producing S. aureus pathogens.
迫切需要开发针对生物膜产生的金黄色葡萄球菌（S. aureus）的治疗方法。因此，我们研究小组重新关注了精油（EOs），特别是香芹酚、芳樟醇和丁子香酚。
采用表型和基因型方法评估了从乳腺炎奶牛中分离的金黄色葡萄球菌菌株的多药耐药和多种毒力特征，以及生物膜的产生。使用体外和分子对接研究测试了 EOs 的抗菌和抗生物膜活性。此外，使用棋盘法检测了常用抗生素与测试 EOs 之间的相互作用。
我们发现，我们所有的分离株（n=37）均为生物膜性耐甲氧西林金黄色葡萄球菌（MRSA）产生者，40.5%为万古霉素耐药金黄色葡萄球菌（VRSA）。不幸的是，73%和 43.2%回收的 MRSA 分离株分别表现出多药耐药（MDR）和多种毒力模式。测试的 EOs 的抗菌活性与表型评估的抗生物膜活性和分子对接研究相符。芳樟醇显示出最高的抗菌和抗生物膜活性，其次是香芹酚和丁子香酚 EOs。幸运的是，在 FICI 值≤0.5 时，研究的 EOs 与甲氧西林或万古霉素之间检测到协同相互作用。用任何一种测试的 EOs 处理后，13 株分离株的抗菌耐药模式均转变为敏感表型。通过将研究的 EOs 与现有的抗菌药物联合使用，可以避免对耐药性金黄色葡萄球菌引起的牛乳腺炎的治疗失败。
我们希望我们的发现可以转化为针对生物膜产生的金黄色葡萄球菌病原体的新药物制剂的配方。
Zotero 插件
