Almarsomy Dhuha H, Al-Khayat Fadia A, Al-Taee Lamis A
Department of Conservative and Aesthetic Dentistry, Baghdad College of Dentistry, University of Baghdad, Baghdad, Iraq.
Department of Basic Sciences, Baghdad College of Dentistry, University of Baghdad, Baghdad, Iraq.
Heliyon. 2023 Sep 23;9(10):e20310. doi: 10.1016/j.heliyon.2023.e20310. eCollection 2023 Oct.
To evaluate the preventive and therapeutic effects of CO laser and MI paste plus on intact and demineralized enamel surfaces and their impact on bacterial adhesion. Methods: 160 enamel slabs were prepared and randomly allocated into two main groups; sound and demineralized enamel (n = 80 per group), in which specimens were immersed in a demineralizing solution (50 mM acetic acid, pH 4.5) for 72 h at 37 °C. Each group was further divided into four subgroups (n = 20); the control (un treated surfaces), surfaces treated by CO laser, MI paste plus (Recaldent™, GC corporation/Germany), and those received a combination of CO and MI paste plus. biofilm was isolated, quantified, and then applied on treated enamel surfaces and incubated anaerobically for 24 h and then quantified by colony-forming unit (CFU). Meanwhile, surface changes were assessed by Vickers microhardness and Scanning Electron Microscope combined with Energy-Dispersive X-Ray Spectroscopy (SEM-EDX). Results: The combined use of CO laser followed by MI paste plus significantly (p < 0.000) enhanced surface microhardness of sound and demineralized enamel with a significant reduction in bacterial counts. However, each technique alone was beneficial as they exhibited higher microhardness with lower bacterial viability in comparison to the control. The treatment of demineralized enamel surfaces with MI paste significantly reduced the number of bacterial colonies with the presence of dispersed mineral deposits over the surface.
The combined use of CO laser and MI paste plus was effective as a preventive and/or therapeutic measures in enhancing surface properties of enamel and reducing the bacterial viability.
评估CO激光和含MI糊剂对完整和脱矿釉质表面的预防和治疗效果及其对细菌黏附的影响。方法：制备160块釉质片，随机分为两个主要组；健康釉质和脱矿釉质（每组n = 80），其中样本在37°C下于脱矿溶液（50 mM乙酸，pH 4.5）中浸泡72小时。每组进一步分为四个亚组（n = 20）；对照组（未处理表面）、CO激光处理表面、含MI糊剂（Recaldent™，GC公司/德国）处理表面以及接受CO激光和含MI糊剂联合处理的表面。分离生物膜、进行定量，然后将其应用于处理过的釉质表面，厌氧培养24小时，然后通过菌落形成单位（CFU）进行定量。同时，通过维氏显微硬度以及扫描电子显微镜结合能量色散X射线光谱仪（SEM - EDX）评估表面变化。结果：先使用CO激光再使用含MI糊剂的联合处理显著（p < 0.000）提高了健康釉质和脱矿釉质的表面显微硬度，并显著减少了细菌数量。然而，单独使用每种技术也都有益，因为与对照组相比，它们表现出更高的显微硬度和更低的细菌活力。用含MI糊剂处理脱矿釉质表面显著减少了细菌菌落数量，且表面存在分散的矿物质沉积。结论：CO激光和含MI糊剂的联合使用作为一种预防和/或治疗措施，在增强釉质表面性能和降低细菌活力方面是有效的。
