Ferreira Raul L, Jr Walter Muniz, Souza Louise E A, Navarro Hector M C, de Mello Lucas R, Mastelaro Valmor R, Sales Tasso O, Barbosa Cintya D A E S, Ribeiro Adriana S, da Silva Emerson R, Landell Melissa F, de Oliveira Italo N
Instituto de Física, Universidade Federal de Alagoas, 57072-970 Maceió-AL, Brazil.
Departmento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, SP, Brazil.
ACS Appl Bio Mater. 2023 Oct 16;6(10):4345-4357. doi: 10.1021/acsabm.3c00541. Epub 2023 Oct 4.
The emergence of drug-resistant pathogenic microorganisms has become a public health concern, with demand for strategies to suppress their proliferation in healthcare facilities. The present study investigates the physicochemical and antimicrobial properties of carbon dots (CD-MR) derived from the methyl red azo dye. The morphological and structural analyses reveal that such carbon dots present a significant fraction of graphitic nitrogen in their structures, providing a wide emission range. Based on their low cytotoxicity against mammalian cells and tunable photoluminescence, these carbon dots are applied to bioimaging living cells. The possibility of using CD-MR to generate reactive oxygen species (ROS) is also analyzed, and a high singlet oxygen quantum efficiency is verified. Moreover, the antimicrobial activity of CD-MR is analyzed against pathogenic microorganisms , , and . Kirby-Bauer susceptibility tests show that carbon dots synthesized from methyl red possess antimicrobial activity upon photoexcitation at 532 nm. The growth inhibition of from CD-MR photosensitization is investigated. Our results show that N-doped carbon dots synthesized from methyl red efficiently generate ROS and possess a strong antimicrobial activity against healthcare-relevant pathogens.
耐药致病微生物的出现已成为一个公共卫生问题，人们需要采取策略来抑制它们在医疗机构中的增殖。本研究调查了源自甲基红偶氮染料的碳点（CD-MR）的物理化学和抗菌特性。形态和结构分析表明，此类碳点在其结构中存在大量的石墨氮，具有较宽的发射范围。基于其对哺乳动物细胞的低细胞毒性和可调谐的光致发光特性，这些碳点被应用于活细胞的生物成像。还分析了使用CD-MR产生活性氧（ROS）的可能性，并验证了其较高的单线态氧量子效率。此外，还分析了CD-MR对致病微生物、和的抗菌活性。 Kirby-Bauer药敏试验表明，由甲基红合成的碳点在532 nm光激发下具有抗菌活性。研究了CD-MR光致敏对的生长抑制作用。我们的结果表明，由甲基红合成的N掺杂碳点能有效产生活性氧，对与医疗保健相关的病原体具有很强的抗菌活性。
