Department of Ceramic, Materials and Energy Research Center, Karaj, Iran.
School of Metallurgy and Materials Engineering, Iran University of Science and Technology, Tehran, Iran.
Odontology. 2024 Apr;112(2):408-427. doi: 10.1007/s10266-023-00853-6. Epub 2023 Oct 4.
Zirconia-based dental implants are in direct contact with living tissues and any improvements in their bioactivity and adhesion to the tissues are highly welcome. In this study, different ratios of barium titanate (BT) were added to 3 mol% yttria-stabilized zirconia (3YSZ) through conventional sintering. The laser-texturing technique was also conducted to improve the biological performance of 3YSZ ceramics. The composition and the surface of the prepared composites were characterized by X-ray diffraction and scanning electron microscopy (SEM), respectively. The roughness and surface wettability of the composites were also measured. Furthermore, MC3T3-E1 pre-osteoblast cells were used for the in vitro experiments. Cell viability was evaluated using a commercial resazurin-based method. Morphology and cellular adhesion were observed using SEM. Based on the results, the laser texturing and the barium titanate content influenced the surface characteristics of the prepared composites. The laser-textured 3YSZ/7 mol% BT composites showed a lower water contact angle compared to the other samples, which indicated superior surface hydrophilicity. The cell viability and cell adhesion of 3YSZ/BT composites increased with the rise in the barium titanate content and laser power. An elongated cell morphology and apatite nucleation were also observed by the BT content. Overall, the laser-treated 3YSZ/5 and 7 mol% BT composites may be promising candidates in hard tissue repair due to their good cell response.
氧化锆基牙科植入物与活组织直接接触，因此非常欢迎提高其生物活性和与组织的粘附性。在这项研究中，通过传统烧结法将不同比例的钛酸钡（BT）添加到 3 摩尔%氧化钇稳定氧化锆（3YSZ）中。还采用激光纹理化技术来改善 3YSZ 陶瓷的生物性能。通过 X 射线衍射和扫描电子显微镜（SEM）分别对制备的复合材料的组成和表面进行了表征。还测量了复合材料的粗糙度和表面润湿性。此外，还使用 MC3T3-E1 前成骨细胞进行了体外实验。使用商业的 Resazurin 法评估细胞活力。使用 SEM 观察形态和细胞粘附。根据结果，激光纹理和钛酸钡含量影响了所制备复合材料的表面特性。与其他样品相比，激光纹理化的 3YSZ/7 mol% BT 复合材料的水接触角较低，表明表面亲水性更好。3YSZ/BT 复合材料的细胞活力和细胞粘附随钛酸钡含量和激光功率的增加而增加。还观察到 BT 含量导致细胞形态拉长和磷灰石成核。总的来说，由于具有良好的细胞反应，经激光处理的 3YSZ/5 和 7 mol% BT 复合材料可能是硬组织修复的有前途的候选材料。
