Tricoli J V, Rall L B, Karakousis C P, Herrera L, Petrelli N J, Bell G I, Shows T B
Cancer Res. 1986 Dec;46(12 Pt 1):6169-73.
The insulin-like growth factors I and II (IGF-I and -II) are proteins which stimulate cell proliferation and are important in normal human growth and development. They are coded for by separate genes and bind to specific cell surface receptors, eliciting a mitogenic response. IGFs are secreted by several cell lines derived from adult tumors. We have examined a number of human adult tumors for IGF messenger RNA (mRNA) expression and found IGF-II mRNA levels were consistently elevated in two types, colon carcinoma and liposarcoma. Adult colonic mucosa contains low levels of IGF-I and -II mRNA while several colon tumors, particularly of rectal and rectosigmoid origin, contained significantly elevated levels of IGF-II message. Over 90% of liposarcomas examined contained greatly elevated levels of IGF-II mRNA while control tissue (adipose) contained very low or undetectable IGF mRNA levels. Many of these tumors also contained elevated IGF-I mRNA levels. Northern analysis of these RNAs revealed differences in the abundance and sizes of IGF transcripts compared to other normal and malignant tissues known to express IGF.
胰岛素样生长因子I和II（IGF-I和-II）是刺激细胞增殖的蛋白质，在人类正常生长发育中起重要作用。它们由不同的基因编码，并与特定的细胞表面受体结合，引发有丝分裂反应。IGF由源自成人肿瘤的多种细胞系分泌。我们检测了多种人类成人肿瘤中的IGF信使核糖核酸（mRNA）表达，发现IGF-II mRNA水平在结肠癌和脂肪肉瘤这两种肿瘤类型中持续升高。成人结肠黏膜中IGF-I和-II mRNA水平较低，而一些结肠肿瘤，特别是起源于直肠和直肠乙状结肠的肿瘤，其IGF-II信使水平显著升高。超过90%的检测脂肪肉瘤中IGF-II mRNA水平大幅升高，而对照组织（脂肪）中IGF mRNA水平非常低或无法检测到。许多这些肿瘤中IGF-I mRNA水平也升高。对这些RNA的Northern分析显示，与已知表达IGF的其他正常和恶性组织相比，IGF转录本在丰度和大小上存在差异。
