Apsley Abner T, Lee Sun Ah, Bhat Aarti C, Rush Jonathan, Almeida David M, Cole Steven W, Shalev Idan
Department of Biobehavioral Health, The Pennsylvania State University, University Park, PA, USA; Department of Molecular, Cellular, and Integrative Biosciences, The Pennsylvania State University, University Park, PA, USA.
Department of Human Development and Family Studies, The Pennsylvania State University, USA.
Brain Behav Immun. 2024 Jan;115:80-88. doi: 10.1016/j.bbi.2023.09.025. Epub 2023 Oct 3.
Affective reactivity to stress is a person-level measurement of how well an individual copes with daily stressors. A common method of measuring affective reactivity entails the estimation of within-person differences of either positive or negative affect on days with and without stressors present. Individuals more reactive to common stressors, as evidenced by affective reactivity measurements, have been shown to have increased levels of circulating pro-inflammatory markers. While affective reactivity has previously been associated with inflammatory markers, the upstream mechanistic links underlying these associations are unknown. Using data from the Midlife in the United States (MIDUS) Refresher study (N = 195; 52% female; 84% white), we quantified daily stress processes over 10 days and determined individuals' positive and negative affective reactivities to stressors. We then examined affective reactivity association with peripheral blood mononuclear cell (PBMC) gene expression of the immune-related conserved transcriptional response to adversity. Results indicated that individuals with a greater decrease in positive affect to daily stressors exhibited heightened PBMC JUNB expression after Bonferroni corrections (p-adjusted < 0.05). JUNB encodes a protein that acts as a transcription factor which regulates many aspects of the immune response, including inflammation and cell proliferation. Due to its critical role in the activation of macrophages and maintenance of CD4+ T-cells during inflammation, JUNB may serve as a potential upstream mechanistic target for future studies of the connection between affective reactivity and inflammatory processes. Overall, our findings provide evidence that affective reactivity to stress is associated with levels of immune cell gene expression.
对应激的情感反应性是对个体应对日常应激源能力的一种个人层面的衡量。测量情感反应性的一种常见方法是估计在有和没有应激源的日子里个体积极或消极情绪的个体内差异。情感反应性测量表明，对常见应激源反应更强的个体，其循环促炎标志物水平有所升高。虽然情感反应性以前与炎症标志物有关，但这些关联背后的上游机制联系尚不清楚。利用美国中年（MIDUS）复习研究的数据（N = 195；52%为女性；84%为白人），我们对10天内的日常应激过程进行了量化，并确定了个体对应激源的积极和消极情感反应性。然后，我们研究了情感反应性与外周血单核细胞（PBMC）对逆境的免疫相关保守转录反应基因表达之间的关联。结果表明，在进行Bonferroni校正后，对日常应激源积极情绪下降幅度更大的个体，其PBMC中JUNB的表达升高（p校正<0.05）。JUNB编码一种作为转录因子的蛋白质，该转录因子调节免疫反应的许多方面，包括炎症和细胞增殖。由于其在炎症期间巨噬细胞激活和CD4+T细胞维持中的关键作用，JUNB可能作为未来研究情感反应性与炎症过程之间联系的潜在上游机制靶点。总体而言，我们的研究结果提供了证据，表明对应激的情感反应性与免疫细胞基因表达水平有关。
