Katila M L, Ojanen T H, Mäntyjärvi R A
Clin Allergy. 1986 Sep;16(5):459-67. doi: 10.1111/j.1365-2222.1986.tb01981.x.
A group of dairy farmers studied 6 years earlier in a field survey was re-surveyed for respiratory symptoms, occupational capability and the presence of antibodies against environmental micro-organisms. Specific IgG antibodies to Micropolyspora faeni, Thermoactinomyces vulgaris, Aspergillus umbrosus and Aspergillus fumigatus were measured by ELISA from the serum samples obtained in the two surveys. Antibody titres remained constant in 70% of both farmers and controls, but where changes took place, the titres against the actinomycete antigens tended to rise, whereas both increases and decreases were detected equally against the Aspergillus antigens. The titre of specific antibody to any of the four micro-organisms, when measured from a single serum specimen, seemed to be of little diagnostic value. Observed changes however, were more diagnostic, in that a fall in titre, especially against the Aspergillus antigens, was closely associated with a definite decrease in exposure, such as after retirement. Increased titres occurred in farmers with continued exposure, and those against the actinomycetes were associated with the appearance of symptoms in previously symptom-free individuals. In a case of farmer's lung which developed in this population during the follow-up period, significant increases were detectable against T. vulgaris and M. faeni.
一组6年前在实地调查中研究过的奶农再次接受了呼吸症状、职业能力以及针对环境微生物的抗体检测。通过酶联免疫吸附测定法（ELISA），从两次调查获得的血清样本中检测了针对嗜热放线多孢菌、普通嗜热放线菌、荫生曲霉和烟曲霉的特异性IgG抗体。70%的奶农和对照组的抗体滴度保持不变，但在发生变化的情况下，针对放线菌抗原的滴度往往会上升，而针对曲霉抗原的滴度则有升有降。从单个血清样本中检测到的针对这四种微生物中任何一种的特异性抗体滴度似乎诊断价值不大。然而，观察到的变化更具诊断意义，因为滴度下降，尤其是针对曲霉抗原的滴度下降，与接触量的明显减少密切相关，比如退休后。持续接触的奶农抗体滴度升高，而针对放线菌的滴度升高与之前无症状个体出现症状有关。在随访期间该人群中发生的一例农民肺病例中，可检测到针对普通嗜热放线菌和嗜热放线多孢菌的抗体滴度显著升高。
