行为过程中神经动力学的深部脑光学记录。
Deep-brain optical recording of neural dynamics during behavior.
作者信息
Zhou Zhe Charles, Gordon-Fennell Adam, Piantadosi Sean C, Ji Na, Smith Spencer LaVere, Bruchas Michael R, Stuber Garret D
机构信息
Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98195, USA; Center for Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA 98195, USA.
Department of Physics, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
出版信息
Neuron. 2023 Dec 6;111(23):3716-3738. doi: 10.1016/j.neuron.2023.09.006. Epub 2023 Oct 6.
In vivo fluorescence recording techniques have produced landmark discoveries in neuroscience, providing insight into how single cell and circuit-level computations mediate sensory processing and generate complex behaviors. While much attention has been given to recording from cortical brain regions, deep-brain fluorescence recording is more complex because it requires additional measures to gain optical access to harder to reach brain nuclei. Here we discuss detailed considerations and tradeoffs regarding deep-brain fluorescence recording techniques and provide a comprehensive guide for all major steps involved, from project planning to data analysis. The goal is to impart guidance for new and experienced investigators seeking to use in vivo deep fluorescence optical recordings in awake, behaving rodent models.
体内荧光记录技术在神经科学领域取得了具有里程碑意义的发现，有助于深入了解单细胞和回路水平的计算如何介导感觉处理并产生复杂行为。虽然人们对皮质脑区的记录给予了很多关注，但深部脑区荧光记录更为复杂，因为它需要采取额外措施才能光学进入难以到达的脑核。在这里，我们讨论了有关深部脑区荧光记录技术的详细注意事项和权衡，并为从项目规划到数据分析的所有主要步骤提供了全面指南。目标是为寻求在清醒的行为啮齿动物模型中使用体内深部荧光光学记录的新老研究人员提供指导。
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