Public Research Center, Hainan Medical College, Haikou, China.
Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
PeerJ. 2023 Oct 4;11:e16156. doi: 10.7717/peerj.16156. eCollection 2023.
Root-associated microbes of the mangrove trees play important roles in protecting and maintaining mangrove ecosystems. At present, most of our understanding of mangrove root-related microbial diversity is obtained from specific mangrove species in selected geographic regions. Relatively little is known about the composition of the bacterial microbiota existing in disparate mangrove species microenvironments, particularly the relationship among different mangrove species in tropical environments.
We collected the root, rhizosphere soil, and non-rhizosphere soil of four mangrove trees (, , and ) and detected the 16S rRNA gene by a conventional PCR. We performed high throughput sequencing using Illumina Novaseq 6000 platform (2 × 250 paired ends) to investigate the bacterial communities related with the different mangrove species.
We analyzed the bacterial diversity and composition related to the diverse ecological niches of mangrove species. Our data confirmed distinct distribution patterns of bacterial communities in the three rhizocompartments of the four mangrove species. Microbiome composition varied with compartments and host mangrove species. The bacterial communities between the endosphere and the other two compartments were distinctly diverse independent of mangrove species. The large degree of overlap in critical community members of the same rhizocompartment across distinct mangrove species was found at the phylum level. Furthermore, this is the first report of found in mangrove environments. In conclusion, understanding the complicated host-microbe associations in different mangrove species could lay the foundation for the exploitation of the microbial resource and the production of secondary metabolites.
红树林树木的根相关微生物在保护和维持红树林生态系统方面发挥着重要作用。目前，我们对红树林根相关微生物多样性的大部分了解都是从选定地理区域的特定红树林物种中获得的。对于不同红树林物种微环境中存在的细菌微生物群落的组成，特别是热带环境中不同红树林物种之间的关系，我们知之甚少。
我们收集了四种红树林树木（、、和）的根、根际土壤和非根际土壤，并通过常规 PCR 检测 16S rRNA 基因。我们使用 Illumina Novaseq 6000 平台（2×250 对端）进行高通量测序，以研究与不同红树林物种相关的细菌群落。
我们分析了与红树林物种不同生态位相关的细菌多样性和组成。我们的数据证实了细菌群落在四种红树林物种的三个根际区的不同分布模式。微生物组组成随隔间和宿主红树林物种而变化。内共生体和其他两个隔间之间的细菌群落明显不同于红树林物种。在门水平上，发现同一根际区的关键群落成员具有很大程度的重叠。此外，这是首次在红树林环境中发现。总之，了解不同红树林物种中复杂的宿主-微生物关联可以为微生物资源的开发和次生代谢产物的生产奠定基础。
