Boutin B K, Townsend S F, Scarpino P V, Twedt R M
Appl Environ Microbiol. 1979 Mar;37(3):647-53. doi: 10.1128/aem.37.3.647-653.1979.
To determine possible pathogenesis of Vibrio parahaemolyticus-host-organ system interactions, studies of invasiveness were made by a direct fluorescent-antibody method. Broth cultures of live cells isolated from seafish or symptomatic humans were inoculated separately into ligated ileal loops of young New Zealand white rabbits. After suitable incubation, rabbits were sacrificed, and ileal loops and tissue specimens were aseptically removed. Ileal loops were prepared and stained with specific fluorescein-tagged antibody, and organ specimens were cultured for isolation of the inoculated Vibrio strain. All strains tested penetrated into the lamina propria of the ileum and were isolated from the cultured tissue specimens, indicating that the organism is capable of more than a superficial colonization of the gut. The presence of Vibrio in cultured tissue specimens suggests invasion of deeper tissue by either the lymphatic or the circulatory system.
为了确定副溶血性弧菌与宿主器官系统相互作用的可能发病机制，采用直接荧光抗体法对侵袭性进行了研究。将从海鱼或有症状的人体中分离出的活细胞肉汤培养物分别接种到年轻新西兰白兔的结扎回肠袢中。经过适当的孵育后，处死兔子，并无菌取出回肠袢和组织标本。制备回肠袢并用特异性荧光素标记抗体染色，对器官标本进行培养以分离接种的弧菌菌株。所有测试菌株均穿透回肠固有层，并从培养的组织标本中分离出来，这表明该菌能够在肠道进行不止是表面的定植。培养组织标本中弧菌的存在表明其通过淋巴系统或循环系统侵入更深层组织。
