Carruthers M M
J Infect Dis. 1975 Nov;132(5):555-60. doi: 10.1093/infdis/132.5.555.
In an investigation of the effect of Vibrio parahaemolyticus on epithelial cells in vitro, HeLa cells in slide culture chambers were inoculated with washed bacteria and several bacterial preparations. Preparations tested included live and heat-killed whole cells, unconcentrated culture filtrates, whole-cell lysates and their supernatant fluids, and supernatant fluids taken from HeLa cell monolayers previously inoculated with live bacteria. Slide cultures were evaluated at 3 and 5 hr by light microscopy and, in a few instances, by electron microscopy (live preparations) or at 24 hr by light microscopy (nonviable preparations). Live V. parahaemolyticus had a rapid cytotoxic effect that did not appear to be due to cellular invasion. The nonviable preparations had no effect on HeLa cells. The results demonstrate that V. parahaemolyticus is rapidly cytotoxic to epithelial cells in vitro and suggest that the presence of live organisms may be required for its enteropathogenicity.
在一项关于副溶血性弧菌对体外上皮细胞影响的研究中，将洗涤后的细菌和几种细菌制剂接种到玻片培养室中的HeLa细胞上。测试的制剂包括活的和热灭活的全细胞、未浓缩的培养滤液、全细胞裂解物及其上清液，以及取自先前接种活细菌的HeLa细胞单层的上清液。玻片培养物在3小时和5小时通过光学显微镜进行评估，在少数情况下，通过电子显微镜（活制剂）进行评估，或在24小时通过光学显微镜（无活力制剂）进行评估。活的副溶血性弧菌具有快速的细胞毒性作用，这似乎不是由于细胞侵袭所致。无活力的制剂对HeLa细胞没有影响。结果表明，副溶血性弧菌在体外对上皮细胞具有快速的细胞毒性作用，并表明其肠道致病性可能需要活生物体的存在。
