Gromska W, Mayer H
Eur J Biochem. 1976 Feb 16;62(2):391-9. doi: 10.1111/j.1432-1033.1976.tb10171.x.
Lipopolysaccharides of qualitatively identical but quantitatively different sugar composition were extracted from Proteus mirabilis strain 1959. The lipopolysaccharide with the higher percentage of typical O-specific constituents was subjected to partial acid hydrolysis. An oligosaccharide B22 was separated by paper chromatography and electrophoresis. It was found to be composed of equimolar amounts of D-galacturonic acid, D-galactosamine and L-lysine. Dinitrophenylation of the oligosaccharide as well as of the genuine lipopolysaccharide afforded xi-dinitrophenyl-L-lysine after acid hydrolysis, showing that lysine was linked to the disaccharide via its alpha-amino group. Further studies including the Morgan-Elson and Elson-Morgan reactions, NaBH4-reduction, hydrazinolysis and periodate oxidation revealed the structure of oligosaccharide B22 as D-galacturonyl-(1 leads to 4)-D-galactosamine with lysine attached to the carboxylic group of galacturonic acid via its alpha-amino group. Judged from its high inhibition capacity this oligosaccharide has to be considered as an essential part of the serological determinant of Proteus mirabilis 1959. The frequent occurrence of lysine and galacturonic acid in Proteus mirabilis O-serogroups and their possible significance for the respective serological specificities are discussed.
从奇异变形杆菌1959菌株中提取了糖组成在性质上相同但数量不同的脂多糖。将具有较高比例典型O-特异性成分的脂多糖进行部分酸水解。通过纸色谱法和电泳分离出一种寡糖B22。发现它由等摩尔量的D-半乳糖醛酸、D-半乳糖胺和L-赖氨酸组成。对该寡糖以及真正的脂多糖进行二硝基苯化，酸水解后得到α-二硝基苯基-L-赖氨酸，表明赖氨酸通过其α-氨基与二糖相连。包括摩根-埃尔森反应和埃尔森-摩根反应、硼氢化钠还原、肼解和高碘酸盐氧化在内的进一步研究揭示了寡糖B22的结构为D-半乳糖醛酸-(1→4)-D-半乳糖胺，赖氨酸通过其α-氨基连接到半乳糖醛酸的羧基上。从其高抑制能力判断，该寡糖必须被视为奇异变形杆菌[1959]血清学决定簇的重要组成部分。文中讨论了赖氨酸和半乳糖醛酸在奇异变形杆菌O血清群中的频繁出现及其对各自血清学特异性的可能意义。
