DeStefano Sabrina, Josyula Aditya, Faust Mondreakest, Fertil Daphna, Lokwani Ravi, Ngo Tran B, Sadtler Kaitlyn
Section on Immunoengineering, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda MD 20892.
bioRxiv. 2023 Aug 17:2023.08.15.553390. doi: 10.1101/2023.08.15.553390.
Upon implantation into a patient, any biomaterial induces a cascade of immune responses that influences the outcome of that device. This cascade depends upon several factors, including the composition of the material itself and the location in which the material is implanted. There is still significant uncertainty around the role of different tissue microenvironments in the immune response to biomaterials and how that may alter downstream scaffold remodeling and integration. In this study, we present a study evaluating the immune response to decellularized extracellular matrix materials within the intraperitoneal cavity, the subcutaneous space, and in a traumatic skeletal muscle injury microenvironment. All different locations induced robust cellular recruitment, specifically of macrophages and eosinophils. The latter was most prominent in the subcutaneous space. Intraperitoneal implants uniquely recruited B cells that may alter downstream reactivity as adaptive immunity has been strongly implicated in the outcome of scaffold remodeling. These data suggest that the location of tissue implants should be taken together with the composition of the material itself when designing devices for downline therapeutics.
植入患者体内后，任何生物材料都会引发一系列免疫反应，这些反应会影响该装置的使用效果。这一系列反应取决于几个因素，包括材料本身的成分以及材料植入的位置。关于不同组织微环境在对生物材料的免疫反应中的作用以及这如何改变下游支架重塑和整合，仍然存在很大的不确定性。在本研究中，我们展示了一项评估在腹腔、皮下空间以及创伤性骨骼肌损伤微环境中对脱细胞细胞外基质材料的免疫反应的研究。所有不同位置都引发了强大的细胞募集，特别是巨噬细胞和嗜酸性粒细胞。后者在皮下空间最为显著。腹腔内植入物独特地募集了B细胞，这可能会改变下游反应性，因为适应性免疫与支架重塑的结果密切相关。这些数据表明，在设计用于后续治疗的装置时，应将组织植入的位置与材料本身的成分综合考虑。
