Department of Urology, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, China; Institute of Cancer and Basic Medicine (IBMC), Chinese Academy of Sciences, Hangzhou, China.
Department of Urology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
Biomol Biomed. 2024 Jan 3;24(1):170-175. doi: 10.17305/bb.2023.9694.
Urinary tract cells respond to bladder distension by releasing adenosine triphosphate (ATP). Patients with interstitial cystitis/bladder pain syndrome (IC/BPS) exhibit elevated urinary ATP levels compared to asymptomatic controls. This study aimed to evaluate the potential of urinary ATP as a non-invasive biomarker for IC/BPS and its correlation with symptom severity. We included 56 patients diagnosed with IC/BPS and 50 asymptomatic controls. Urine samples were collected from both groups. Urinary ATP levels were quantified using the luciferin-luciferase bioluminescence method. The severity of IC/BPS symptoms was assessed using the visual analogue score (VAS), Interstitial Cystitis Symptom Index (ICSI), and Interstitial Cystitis Problem Index (ICPI) from the O'Leary-Sant score. We specifically examined the correlation between symptom scores and urinary ATP levels in IC/BPS patients. Urinary ATP levels were significantly higher in IC/BPS patients compared to the control group (P < 0.0001). There was a significant positive correlation between urinary ATP concentrations and VAS, ICPI, and ICSI scores among IC/BPS patients (P < 0.0001). The threshold value for ATP concentration was set at 56.6 nM, with an area under the receiver operating characteristic (ROC) curve of 0.811 (95% CI 0.730 - 0.892). Our findings indicate that IC/BPS patients excrete elevated amounts of ATP in their urine. This suggests that urinary ATP might serve as a non-invasive biomarker for IC/BPS, with a predictive potential in terms of symptom severity.
尿路细胞通过释放三磷酸腺苷（ATP）对膀胱扩张作出反应。与无症状对照相比，间质性膀胱炎/膀胱疼痛综合征（IC/BPS）患者的尿 ATP 水平升高。本研究旨在评估尿 ATP 作为 IC/BPS 的非侵入性生物标志物的潜力及其与症状严重程度的相关性。我们纳入了 56 名被诊断为 IC/BPS 的患者和 50 名无症状对照。从两组中收集尿液样本。使用荧光素-荧光酶生物发光法定量尿 ATP 水平。使用视觉模拟评分（VAS）、间质性膀胱炎症状指数（ICSI）和 O'Leary-Sant 评分中的间质性膀胱炎问题指数（ICPI）评估 IC/BPS 症状的严重程度。我们特别检查了 IC/BPS 患者症状评分与尿 ATP 水平之间的相关性。与对照组相比，IC/BPS 患者的尿 ATP 水平显著升高（P < 0.0001）。在 IC/BPS 患者中，尿 ATP 浓度与 VAS、ICPI 和 ICSI 评分之间存在显著正相关（P < 0.0001）。ATP 浓度的阈值设定为 56.6 nM，接收器操作特征（ROC）曲线下面积为 0.811（95%CI 0.730-0.892）。我们的研究结果表明，IC/BPS 患者尿液中排泄的 ATP 量增加。这表明尿 ATP 可能作为 IC/BPS 的非侵入性生物标志物，在预测症状严重程度方面具有潜力。
