Seratnahaei Marjan, Eshraghi Seyyed Saeed, Pakzad Parviz, Zahraei-Ramazani Alireza, Yaseri Mehdi
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Arthropod Borne Dis. 2023 Jun 30;17(2):187-196. doi: 10.18502/jad.v17i2.13623. eCollection 2023 Jun.
Larvicidal agents can be produced using microbial resources, which are environmentally friendly, biodegradable, and economical. The study's goal was to evaluate the larvicidal activity of metabolites isolated from (, and ) and () bacterial species against .
Four metabolites isolated from and strains were exanimated for larvicidal activity. The experiments were performed for 24, 48, and 72 hours. 300, 350, 400, 450, 500, 550, and 600 μl of metabolites were added to 100 cc of dechlorinated water. Fourth-stage larvae were placed in dechlorinated water as a control. LC and LC were calculated using toxicity data and analyzed.
All metabolites had a statistically significant influence on mosquito larvae (P< 0.05). At 24, 48, and 72 hours, the LC for N2 () was 417, 386, and 370 ppm, respectively, and the LC was 650, 595, and 561 ppm. Moreover, LC for N4 () was 389, 376, and 347 and LC were 591, 565, and 533 and LC for N5 () was 390, 357, and 341 ppm and LC were 589, 532 ppm. In addition, LC for S921 () was 484, 416, and 382 ppm, and LC was 701, 612, and 574 ppm.
The four bacterial metabolites tested in our study were found to have a notable effect on the mortality rate of larvae, indicating their potential as natural larvicides. This is an effective technique for controlling that has no detrimental environmental impact.
杀幼虫剂可利用微生物资源生产,这些资源环保、可生物降解且经济。该研究的目的是评估从(、和)以及()细菌物种中分离出的代谢产物对的杀幼虫活性。
对从和菌株中分离出的四种代谢产物进行杀幼虫活性检测。实验进行24、48和72小时。将300、350、400、450、500、550和600微升的代谢产物添加到100立方厘米的脱氯水中。将第四阶段幼虫置于脱氯水中作为对照。使用毒性数据计算并分析LC和LC。
所有代谢产物对蚊虫幼虫均有统计学显著影响(P<0.05)。在24、48和72小时时,N2()的LC分别为417、386和370 ppm,LC为650、595和561 ppm。此外,N4()的LC为389、376和347,LC为591、565和533,N5()的LC为390、357和341 ppm,LC为589、532 ppm。另外,S921()的LC为484、416和382 ppm,LC为701、612和574 ppm。
我们研究中测试的四种细菌代谢产物对幼虫的死亡率有显著影响,表明它们作为天然杀幼虫剂的潜力。这是一种控制的有效技术,对环境没有不利影响。