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从红树林环境中分离出的海洋放线菌对[具体对象1]和[具体对象2]的杀幼虫效力。

Larvicidal potency of marine actinobacteria isolated from mangrove environment against and .

作者信息

Balakrishnan S, Santhanam P, Srinivasan M

机构信息

Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620024 India.

Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu 608 502 India.

出版信息

J Parasit Dis. 2017 Jun;41(2):387-394. doi: 10.1007/s12639-016-0812-3. Epub 2016 Jul 6.

Abstract

The marine soil samples were collected from different locations of Parangipettai mangrove ecosystem, Vellar estuary, southeast coast of India. Totally 30 different marine actinobacteria were isolated by serial dilution plate technique on starch casein agar medium. The isolated actinobacteria were investigated for their larvicidal activity against and mosquitoes. , , , and were identified as potential biocide producers. Based on the antimicrobial activity, five strains were chosen for larvicidal and pupicidal activity. Among the crude extracts tested, the extract showed significant activity against (LC 1.48 ± 0.09 and LC 3.33 ± 0.22) and (LC 1.30 ± 0.09 and LC 3.13 ± 0.21). Five isolates have shown a most significant mortality rate of the and mosquito larvae. This is an ideal eco-friendly approach for the control of Japanese encephalitis vectors, and malarial vector .

摘要

海洋土壤样本采集自印度东南海岸韦拉尔河口帕兰吉佩泰红树林生态系统的不同地点。通过在淀粉酪蛋白琼脂培养基上采用系列稀释平板技术,共分离出30种不同的海洋放线菌。对分离出的放线菌进行了针对致倦库蚊和白纹伊蚊的杀幼虫活性研究。其中,链霉菌属、游动放线菌属、小单孢菌属、诺卡氏菌属和糖多孢菌属被鉴定为潜在的生物杀灭剂生产者。基于抗菌活性,选择了5个菌株进行杀幼虫和杀蛹活性测试。在所测试的粗提物中(此处原文似乎有缺失信息),提取物对致倦库蚊(LC50为1.48±0.09,LC90为3.33±0.22)和白纹伊蚊(LC50为1.30±0.09,LC90为3.13±0.21)显示出显著活性。5个分离株对致倦库蚊和白纹伊蚊幼虫的死亡率最高。这是控制日本脑炎媒介致倦库蚊和疟疾媒介白纹伊蚊的一种理想的生态友好方法。

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