牙髓干细胞通过CCL2诱导的M2巨噬细胞极化加速伤口愈合。

Dental pulp stem cells accelerate wound healing through CCL2-induced M2 macrophages polarization.

作者信息

Yang Zi, Ma Linsha, Du Conglin, Wang Jingsong, Zhang Chunmei, Hu Lei, Wang Songlin

机构信息

Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Capital Medical University, Beijing, China.

Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

出版信息

iScience. 2023 Sep 24;26(10):108043. doi: 10.1016/j.isci.2023.108043. eCollection 2023 Oct 20.

The crosstalk between mesenchymal stem cells (MSCs) and the host immune function plays a key role in the efficiency of tissue regeneration and wound healing. However, the difference in immunological modulation and tissue regeneration function between MSCs from different sources remains unclear. Compared to PDLSCs, BMMSCs, and ADSCs, DPSCs exhibited greater tissue regeneration potential and triggered more M2 macrophages . DPSCs elicited the polarization of M2a macrophages by conditioned medium and transwell assay and exhibited higher expression levels of C-C motif chemokine ligand 2 (CCL2). Specific blocking of CCL2 could significantly inhibit the DPSCs-induced polarization of M2 macrophages. DPSCs promoted wound healing of the palatal mucosa and M2 macrophages polarization , which could be significantly impaired by CCL2-neutralized antibody. Our data indicate that DPSCs exert better tissue regeneration potential and immunoregulatory function by secreting CCL2, which can enhance MSCs-mediated tissue regeneration or wound healing.

间充质干细胞（MSCs）与宿主免疫功能之间的相互作用在组织再生和伤口愈合效率中起关键作用。然而，不同来源的MSCs在免疫调节和组织再生功能方面的差异仍不清楚。与牙周膜干细胞（PDLSCs）、骨髓间充质干细胞（BMMSCs）和脂肪间充质干细胞（ADSCs）相比，牙髓干细胞（DPSCs）表现出更大的组织再生潜力，并能诱导更多的M2巨噬细胞。通过条件培养基和Transwell实验，DPSCs诱导M2a巨噬细胞极化，并表现出更高水平的C-C基序趋化因子配体2（CCL2）表达。特异性阻断CCL2可显著抑制DPSCs诱导的M2巨噬细胞极化。DPSCs促进腭黏膜伤口愈合和M2巨噬细胞极化，而CCL2中和抗体可显著削弱这种作用。我们的数据表明，DPSCs通过分泌CCL2发挥更好的组织再生潜力和免疫调节功能，这可以增强MSCs介导的组织再生或伤口愈合。