College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
Cells. 2023 Sep 29;12(19):2386. doi: 10.3390/cells12192386.
Pear ring rot disease is an important branch disease, caused by . With the discovery of fungal viruses, the use of their attenuated properties for biological control provides a new strategy for the biological control of fungal disease. RNA silencing is a major antiviral defense mechanism in plants, insects, and fungi. Viruses encode and utilize RNA silencing suppressors to suppress host defenses. Previous studies revealed that Botryosphaeria dothidea chrysovirus 1 (BdCV1) exhibited weak pathogenicity and could activate host gene silencing by infecting . The aim of our study was to investigate whether BdCV1 can encode a silencing suppressor and what effect it has on the host. In this study, the capability of silencing inhibitory activity of four BdCV1-encoded proteins was analyzed, and the P3 protein was identified as a BdCV1 RNA silencing suppressor in the exotic host line 16c. In addition, we demonstrated that P3 could inhibit local silencing, block systemic RNA silencing, and induce the necrosis reaction of tobacco leaves. Furthermore, overexpression of P3 could slow down the growth rate and reduce the pathogenicity of , and to some extent affect the expression level of RNA silencing components and virus-derived siRNAs (vsiRNAs). Combined with transcriptomic analysis, P3 had an effect on the gene expression and biological process of . The obtained results provide new theoretical information for further study of interaction between BdCV1 P3 as a potential silencing suppressor and .
串珠镰孢菌腐烂病是一种重要的枝枯病，由 引起。随着真菌病毒的发现，利用其减毒特性进行生物防治为真菌病害的生物防治提供了新策略。RNA 沉默是植物、昆虫和真菌的主要抗病毒防御机制。病毒编码并利用 RNA 沉默抑制剂来抑制宿主防御。先前的研究表明，球腔菌生呼肠孤病毒 1（BdCV1）表现出较弱的致病性，并通过感染 来激活宿主基因沉默。本研究旨在探讨 BdCV1 是否能编码沉默抑制子，以及它对宿主的影响。在本研究中，分析了四个 BdCV1 编码蛋白的沉默抑制活性，发现 P3 蛋白是外来宿主 16c 中的 BdCV1 RNA 沉默抑制剂。此外，我们证明 P3 可以抑制局部沉默，阻断系统 RNA 沉默，并诱导烟草叶片的坏死反应。此外，P3 的过表达可以减缓 的生长速度并降低其致病性，在一定程度上影响 RNA 沉默成分和病毒衍生 siRNAs（vsiRNAs）的表达水平。结合转录组分析，P3 对 BdCV1 P3 作为潜在沉默抑制剂与 的相互作用的基因表达和生物学过程有影响。研究结果为进一步研究 BdCV1 P3 作为潜在沉默抑制子与 的相互作用提供了新的理论信息。
